Effect of nicotine from tobacco root exudates on chemotaxis, growth, biocontrol efficiency, and colonization by Pseudomonas aeruginosa NXHG29

Accumulated evidence suggests that root exudates have a major role in mediating plant–microbe interactions in the rhizosphere. Here, we characterized tobacco root exudates (TREs) by GC–MS and nicotine, scopoletin, and octadecane were identified as three main components of TREs. Qualitative and quant...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2018-07, Vol.111 (7), p.1237-1257
Main Authors: Ma, Li, Zheng, Shuai Chao, Zhang, Ti Kun, Liu, Zi Yi, Wang, Xue Jian, Zhou, Xing Kui, Yang, Cheng Gang, Duo, Jin Ling, Mo, Ming He
Format: Article
Language:English
Subjects:
Antagonism
Bacteria
Biological control
Biomedical and Life Sciences
Black shank
Carbon sources
Chemotaxis
Chemotaxis - drug effects
Colonization
Dilution
Efficiency
Exudates
Exudation
Gas Chromatography-Mass Spectrometry
Greenhouse effect
Greenhouses
Life Sciences
Medical Microbiology
Microbiology
Nicotiana - chemistry
Nicotine
Nicotine - chemistry
Nicotine - isolation & purification
Nicotine - pharmacology
Original Paper
Plant Diseases - microbiology
Plant Exudates - chemistry
Plant Exudates - isolation & purification
Plant Exudates - pharmacology
Plant Roots - chemistry
Plant Sciences
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - physiology
Rhizosphere
Roots
Soil Science & Conservation
Tobacco
Wilt
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Summary:Accumulated evidence suggests that root exudates have a major role in mediating plant–microbe interactions in the rhizosphere. Here, we characterized tobacco root exudates (TREs) by GC–MS and nicotine, scopoletin, and octadecane were identified as three main components of TREs. Qualitative and quantitative chemotaxis assays revealed that Pseudomonas aeruginosa NXHG29 with antagonistic activity displayed positive chemotactic responses towards TREs and their three main components (nicotine, scopoletin, octadecane) and its enhanced chemotaxis were induced by these substances in a concentration-dependent manner. Furthermore, following GC–MS and chemotaxis analysis, nicotine was selected as the target for evaluation of the effect on NXHG29 regarding antagonism, growth, root colonization and biocontrol efficiency. Results of in vitro studies showed that nicotine as a sole carbon source could enhance growth of NXHG29 and significantly increased the antagonism of NXHG29. We also demonstrated that nicotine exerted enhancing effects on the colonization ability of NXHG29 on tobacco roots by combining CLSM observations with investigation of population level dynamics by selective dilution plating method. Results from greenhouse experiments suggested nicotine exhibited stimulatory effects on the biocontrol efficiency of NXHG29 against bacterial wilt and black shank on tobacco. The stimulatory effect of nicotine was affected by the concentration and timing of nicotine application and further supported by the results of population level of NXHG29 on tobacco roots. This is the first report on the enhancement effect of nicotine from TREs on an antagonistic bacterium for its root colonization, control of soil-borne pathogens, regarding the chemotaxis and in vitro antagonism and growth.
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-018-1035-7