Chemical characteristics, antioxidant and anticancer potential of sulfated polysaccharides from Chlamydomonas reinhardtii

The sulfated polysaccharides (SPs) from Chlamydomonas reinhardtii (Cr) were isolated by hot water method using 80% alcohol and semi purified by anion-exchange column chromatography. The chemical analysis of the extract showed 78% carbohydrates, 18% reducing sugars, 60% non-reducing sugars, 2% protei...

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Bibliographic Details
Published in:Journal of applied phycology 2018-06, Vol.30 (3), p.1641-1653
Main Authors: Kamble, Priyanka, Cheriyamundath, Sanith, Lopus, Manu, Sirisha, V. L.
Format: Article
Language:English
Subjects:
Alcohols
Anion exchanging
Anions
Anticancer properties
Antioxidants
Antitumor activity
Apoptosis
Biomedical and Life Sciences
Breast cancer
Cancer
Capacity
Carbohydrates
Cells
Chelation
Chemical analysis
Chlamydomonas reinhardtii
Column chromatography
Deoxyribonucleic acid
DNA
Ecology
Freshwater & Marine Ecology
Hot water
Hydroxyl radicals
Life Sciences
Plant Physiology
Plant Sciences
Polysaccharides
Proliferation
Proteins
Saccharides
Scavenging
Sugar
Sulfates
Uronic acid
Water purification
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Summary:The sulfated polysaccharides (SPs) from Chlamydomonas reinhardtii (Cr) were isolated by hot water method using 80% alcohol and semi purified by anion-exchange column chromatography. The chemical analysis of the extract showed 78% carbohydrates, 18% reducing sugars, 60% non-reducing sugars, 2% protein, 33% sulfate, 39% uronic acid, and 4% ash. The elemental analysis of this C. reinhardtii sulfated polysaccharide-enriched extract (Cr-SPs) showed 53% carbon, 8% hydrogen, and 6% nitrogen. FTIR analysis of Cr-SPs showed characteristic bands of sulfated polysaccharides. Further, the Cr-SPs showed significant hydroxyl radical scavenging activity of 22.29–80.9% at 0.01–1 mg mL −1 , 38–77% of DPPH radical scavenging activity at 0.01–1 mg mL −1 , 9.8–81% ABTS radical scavenging activity, 34.5–67.6% of ferrous chelating ability, and 11.62–75% of total antioxidant capacity. Cr-SPs also showed efficient in vitro anticancer activity. Specifically, they inhibited triple-negative breast cancer cell (MDA-MB-231) proliferation with an IC 50 of 172 μg mL −1 . Concentration-dependent reduction in the number of colonies formed by MDA-MB-231 cells suggested their potential to inhibit the clonal expansion of the cancer cells. Higher concentrations of crude extract were found to disrupt the microtubule networks in these cells. The cells treated with Cr-SPs eventually underwent apoptosis as evidenced by the formation of characteristic DNA ladder. These results indicate that Cr-SPs find promising opportunities for cancer treatment.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-018-1397-2