Phosphatidylserine-exposing blood and endothelial cells contribute to the hypercoagulable state in essential thrombocythemia patients

The mechanisms of thrombogenicity in essential thrombocythemia (ET) are complex and not well defined. Our objective was to explore whether phosphatidylserine (PS) exposure on blood cells and endothelial cells (ECs) can account for the increased thrombosis and distinct thrombotic risks among mutation...

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Bibliographic Details
Published in:Annals of hematology 2018-04, Vol.97 (4), p.605-616
Main Authors: Tong, Dongxia, Yu, Muxin, Guo, Li, Li, Tao, Li, Jihe, Novakovic, Valerie A., Dong, Zengxiang, Tian, Ye, Kou, Junjie, Bi, Yayan, Wang, Jinghua, Zhou, Jin, Shi, Jialan
Format: Article
Language:English
Subjects:
Adult
Aged
Amino Acid Substitution
Blood Platelets - metabolism
Blood Platelets - pathology
Calreticulin - genetics
Calreticulin - metabolism
Cells, Cultured
China - epidemiology
Endothelium, Vascular - metabolism
Endothelium, Vascular - pathology
Erythrocytes - metabolism
Erythrocytes - pathology
Female
Hematology
Human Umbilical Vein Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Janus Kinase 2 - genetics
Janus Kinase 2 - metabolism
Leukocytes - metabolism
Leukocytes - pathology
Male
Medicine
Medicine & Public Health
Microscopy
Middle Aged
Mutation
Oncology
Original Article
Phosphatidylserines - metabolism
Receptors, Thrombopoietin - genetics
Receptors, Thrombopoietin - metabolism
Risk
Surface Properties
Thrombocythemia, Essential - genetics
Thrombocythemia, Essential - metabolism
Thrombocythemia, Essential - pathology
Thrombocythemia, Essential - physiopathology
Thrombosis
Thrombosis - epidemiology
Thrombosis - etiology
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Summary:The mechanisms of thrombogenicity in essential thrombocythemia (ET) are complex and not well defined. Our objective was to explore whether phosphatidylserine (PS) exposure on blood cells and endothelial cells (ECs) can account for the increased thrombosis and distinct thrombotic risks among mutational subtypes in ET. Using flow cytometry and confocal microscopy, we found that the levels of PS-exposing erythrocytes, platelets, leukocytes, and serum-cultured ECs were significantly higher in each ET group [JAK2, CALR, and triple-negative (TN) (all P  
ISSN:0939-5555
1432-0584
DOI:10.1007/s00277-018-3228-6