Heparan sulfate accumulation and perlecan/HSPG2 up-regulation in tumour tissue predict low relapse-free survival for patients with glioblastoma

Glycosaminoglycans are major components of brain extracellular matrix (ECM), although heparan sulfate (HS) contribution in brain physiology and carcinogenesis remains underinvestigated. This study examined HS content and distribution in glioblastoma multiforme (GBM) tissues in the context of potenti...

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Bibliographic Details
Published in:Histochemistry and cell biology 2018-03, Vol.149 (3), p.235-244
Main Authors: Kazanskaya, Galina M., Tsidulko, Alexandra Y., Volkov, Alexander M., Kiselev, Roman S., Suhovskih, Anastasia V., Kobozev, Vyacheslav V., Gaytan, Alexei S., Aidagulova, Svetlana V., Krivoshapkin, Alexei L., Grigorieva, Elvira V.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Brain cancer
Brain Neoplasms - metabolism
Brain Neoplasms - pathology
Brain tumors
Carcinogenesis
Cell Biology
Developmental Biology
Extracellular matrix
Glioblastoma
Glioblastoma - metabolism
Glioblastoma - pathology
Glioma cells
Glycosaminoglycans
Heparan sulfate
Heparan sulfate proteoglycans
Heparan Sulfate Proteoglycans - metabolism
Heparitin Sulfate - analysis
Heparitin Sulfate - metabolism
Humans
Immunohistochemistry
Medical prognosis
Middle Aged
Molecular modelling
Original Paper
Paraffin
Perlecan
Recurrence
Sulfates
Survival Analysis
Syndecan
Transcription
Tumor microenvironment
Tumors
Up-Regulation
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Summary:Glycosaminoglycans are major components of brain extracellular matrix (ECM), although heparan sulfate (HS) contribution in brain physiology and carcinogenesis remains underinvestigated. This study examined HS content and distribution in glioblastoma multiforme (GBM) tissues in the context of potential molecular mechanisms underlying its deregulation in brain tumours. Totally, 42 tissue samples and paraffin-embedded tissues for 31 patients with different prognosis were investigated. HS expression was demonstrated in 50–55% of the GBM tumours by immunohistochemistry (IHC), while almost no HS content was detected in the surrounding paratumourous brain tissues. Heterogeneous HS distribution in the HS-positive tumours was more related to the necrosis or glandular-like brain zones rather than glioma cells with high or low Ki-67 index. According the Kaplan–Meier curves, HS accumulation in glioma cells was associated with low relapse-free survival (RS) of the GBM patients ( p  
ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-018-1631-7