Elevated serum dipeptidyl peptidase IV (CD26, EC 3.4.14.5) activity in experimental liver cirrhosis

Background Dipeptidyl peptidase IV (DPP IV) is a cell surface ectoenzyme widely distributed in the rat body, present on the epithelial cells of the brush border membranes (e.g. bile canaliculi) and on the surface of reactive lymphocytes and fibroblasts. DPP IV has been implicated in hepatocyte–extra...

Full description

Saved in:
Bibliographic Details
Published in:European journal of clinical investigation 2000-09, Vol.30 (9), p.793-797
Main Authors: LAKATOS, P. L, FIRNEISZ, G, BORCSICZKY, D, ZALATNAI, A, SELMECI, L, SZALAY, F
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CD26
Diethylnitrosamine
Dipeptidyl Peptidase 4 - blood
DPP IV
experimental model
fibrogenesis
Gastroenterology. Liver. Pancreas. Abdomen
liver cirrhosis
Liver Cirrhosis, Experimental - blood
Liver Cirrhosis, Experimental - chemically induced
Liver Cirrhosis, Experimental - enzymology
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Male
Medical sciences
Other diseases. Semiology
Rats
Rats, Inbred F344
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Dipeptidyl peptidase IV (DPP IV) is a cell surface ectoenzyme widely distributed in the rat body, present on the epithelial cells of the brush border membranes (e.g. bile canaliculi) and on the surface of reactive lymphocytes and fibroblasts. DPP IV has been implicated in hepatocyte–extracellular matrix interactions, fibroblast activation and proliferation and in T‐cell activation. Aberrant DPP IV expression was found in human liver cirrhosis, and elevated serum DPP IV activity was reported in patients with primary biliary cirrhosis and chronic hepatitis C virus infection. The aim of the study was to examine serum DPP IV activity in experimental liver cirrhosis. Methods Liver cirrhosis was induced by administering diethyl‐nitrosamine, phenobarbital and CCl4 in Fischer‐344 male rats (n = 22). Phenobarbital‐treated (n = 9) and nontreated (n = 9) male rats were used as controls. Serum DPP IV activity was measured using a microplate‐based continuous‐monitoring assay. Recombinant rat DPP IV was used as standard and Gly‐Pro‐PNA was used as substrate. Enzyme activity was given in nmol mL−1 min−1 (U L−1). Results Significantly higher DPP IV activity was found in the sera of rats with experimental liver cirrhosis (39.2 ± 3.7; mean ± SD) compared to phenobarbital‐treated (11 ± 4, P 
ISSN:0014-2972
1365-2362
DOI:10.1046/j.1365-2362.2000.00698.x