Screening of lactic acid bacteria isolated from fermented food as potential probiotics for aquacultured carp and amberjack

This study aimed to isolate lactic acid bacteria from fermented foods and evaluate their probiotic properties for application to aquaculture. Sixty-five bacteria strains were isolated using MRS (de Man, Rogosa & Sharpe) media. Using a double-layer agar method, three strains, GYP 31, L 15 and K-C...

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Bibliographic Details
Published in:Fisheries science 2018-01, Vol.84 (1), p.101-111
Main Authors: Linh, Nguyen Thi Hue, Sakai, Kentaro, Taoka, Yousuke
Format: Article
Language:English
Subjects:
Adhesion tests
Agar
Aquaculture
Bacteria
Biomedical and Life Sciences
Carp
Catalase
Fermented food
Fish & Wildlife Biology & Management
Food
Food Science
Freshwater & Marine Ecology
Freshwater fishes
Gene sequencing
In vitro methods and tests
Intestine
Juices
Lactic acid
Lactic acid bacteria
Life Sciences
Microbiological strains
Mucus
Original Article
pH effects
Probiotics
rRNA 16S
Sodium chloride
Strains (organisms)
Sustainable aquaculture
Yogurt
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Summary:This study aimed to isolate lactic acid bacteria from fermented foods and evaluate their probiotic properties for application to aquaculture. Sixty-five bacteria strains were isolated using MRS (de Man, Rogosa & Sharpe) media. Using a double-layer agar method, three strains, GYP 31, L 15 and K-C2, showed antagonistic activities against all test pathogens belonging to Edwardsiella tarda , Streptococcus dysgalactiae , S. iniae and Lactococcus garvieae . These strains were able to survive in a pH range from 2.0 to 9.0 and at NaCl concentrations of 0, 3 and 5%. In the tolerance test, strain K-C2 displayed higher tolerance than strains GYP 31 and L 15 under the stimulation of acidic pH buffers and artificial gastric-intestinal juices. Thus, strain K-C2 was selected as a probiotic candidate and identified as L. lactis based on the sequences determined in 16S rRNA gene (1438 bp) analysis. It was observed to be ovoid in shape and to be catalase-negative in morphological and biochemical investigations. Strain K-C2 adhered to carp and amberjack intestinal mucus at bacterial densities of 10 10 cfu/ml in in vitro tests with adhesion rates of 62 and 58%, respectively. Thus, L. lactis strain K-C2 was considered to be a potential probiotic candidate for use in sustainable aquaculture.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-017-1150-9