Incremental analysis of vertebral centra can reconstruct the stable isotope chronology of teleost fishes

Isotope analysis has high potential for understanding fish ecology and food‐web structure in aquatic ecosystems. The utility of isotope analysis will be greatly improved if we can reconstruct the chronology of several isotopes at multiple growth stages of individual fish. However, no practical metho...

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Bibliographic Details
Published in:Methods in ecology and evolution 2017-12, Vol.8 (12), p.1755-1763
Main Authors: Matsubayashi, Jun, Saitoh, Yu, Osada, Yutaka, Uehara, Yoshitoshi, Habu, Junko, Sasaki, Tsuyoshi, Tayasu, Ichiro, Kurle, Carolyn
Format: Article
Language:English
Subjects:
87Sr/86Sr
anadromous migration record
Anadromous species
Aquatic ecosystems
Chemical analysis
Chronology
Collagen
Ecological monitoring
Ecology
Fish
Fish migration
fish migration history
Food webs
Fresh water
Freshwater environments
Isotope ratios
Isotopes
Marine ecology
masu salmon
Migration
multi‐isotope chronology
Oncorhynchus masou
otolith
Otoliths
Rivers
Salmon
Seawater
Strontium 87
Sulfur
sulphur stable isotopes
Vertebrae
Water analysis
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Summary:Isotope analysis has high potential for understanding fish ecology and food‐web structure in aquatic ecosystems. The utility of isotope analysis will be greatly improved if we can reconstruct the chronology of several isotopes at multiple growth stages of individual fish. However, no practical methods exist for reconstructing the chronology of light‐element isotopes (e.g. δ13C, δ15N, δ34S, and Δ14C) in teleost fishes. Here, we present and test a new analytical approach for reconstructing the isotopic ratios of light isotopes at multiple life‐stages in teleost fishes. We sampled an anadromous salmon species, masu salmon Oncorhynchus masou (n = 3), along with water from its natal stream and from the ocean. We subdivided the vertebral centra of the salmon equally into 10 sections and extracted bone collagen from each sample. We then measured the stable sulphur isotope ratios of each vertebral section and compared them with δ34S values of the river water and sea water. We also measured the 87Sr/86Sr ratios of otoliths as a reference indicator of salmon migration between fresh water and the ocean. In all samples, the bone section closest to the centre of the centrum had the lowest δ34S values, which were similar to those of fresh water. The δ34S values gradually increased from the centre to marginal sections, finally reaching constant values similar to those of seawater. The 87Sr/86Sr ratios of sagittal otolith sections had significant inter‐individual differences and were consistent with the patterns of variation of the δ34S values of the vertebral sections. Our results show that the vertebral centra of teleost fishes record isotopic information from juvenile to adult life stages. We suggest that our method can provide reproducible isotopic chronology, even in teleost fishes smaller than 50 cm. This method can be used in isoscape studies and in studies of the ecology of marine teleost fishes.
ISSN:2041-210X
2041-210X
DOI:10.1111/2041-210X.12834