Concurrent administration effect of antibiotic and anti-inflammatory drugs on the immunotoxicity of bacterial endotoxins

Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative bacterium that causes a variety of diseases in compromised hosts. Bacterial endotoxins such as lipopolysaccharide (LPS) are the major outer surface membrane components that are present in almost all gram-negative bacteria and act as extremely...

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Bibliographic Details
Published in:International immunopharmacology 2017-11, Vol.52, p.176-184
Main Authors: El Amir, Azza M., Tanious, Dalia G., Mansour, Hanaa A.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - therapeutic use
Anti-inflammatory
Anti-inflammatory agents
Anti-Inflammatory Agents - administration & dosage
Antibiotics
Bacteria
Bacterial endotoxins
Combined treatment
Dexamethasone
Dexamethasone - administration & dosage
Drug Therapy, Combination
Drugs
Dual energy X-ray absorptiometry
Endotoxemia
Endotoxins
Endotoxins - immunology
Gentamicin
Gentamicins - therapeutic use
Gram-negative bacteria
Immunity
Immunity, Innate
Immunoglobulin
Immunoglobulin G
Immunoglobulin M
Immunology
Immunotoxicity
Inflammation
Inflammation - drug therapy
Innate immunity
Leukocytes
Leukocytes (neutrophilic)
Leukocytes - immunology
Lipopolysaccharides
Lipopolysaccharides - immunology
Liver
Lymph nodes
Lymphocytes
Lymphopenia
Male
Mice
Neutrophilia
Organs
P. aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa - immunology
Pseudomonas Infections - drug therapy
Respiratory tract
Spleen
Stimulators
Studies
Thymus
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Summary:Pseudomonas aeruginosa (P. aeruginosa) is a gram-negative bacterium that causes a variety of diseases in compromised hosts. Bacterial endotoxins such as lipopolysaccharide (LPS) are the major outer surface membrane components that are present in almost all gram-negative bacteria and act as extremely strong stimulators of innate immunity and inflammation of the airway. This study was undertaken to determine the effect of combined administration of Gentamicin (GENT) as an antibiotic and Dexamethasone (DEXA) as an anti-inflammatory drug on some immunological and histological parameters. After determination of LD50 of P. aeruginosa, mice groups were injected with DEXA, GENT and lipopolysaccharide alone or in combination. Lipopolysaccharide single injection caused a significant increase of total leukocyte count, lymphocytes, neutrophils and levels of IgM and IgG. DEXA induced an increase of neutrophilia and lymphopenia. Immunological examination demonstrated that combined treatment has a significant effect of decreasing lymphocytes and IgG levels than single treatment does. Histological examination demonstrated that the inflammation of thymus, spleen, lymph node and liver decreases in mice that received combined treatment than those that received individual treatment. Concurrent administration of DEXA and GENT has a great effect on protecting organs against damage in case of endotoxemia. •Bacterial challenge test to determine LD50 of Lipopolysaccharides was done.•Mice groups were injected with Dexamethasone, Garamycin and lipopolysaccharide alone or in combination.•Some immunological and histological parameters were assessed.•Protection effect of concurrent administration of Dexamethasone and Garamycin on organs against damage in case of endotoxemia was noticed.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2017.09.006