Mitsuaria sp. and Burkholderia sp. from Arabidopsis rhizosphere enhance drought tolerance in Arabidopsis thaliana and maize (Zea mays L.)

Background and aims Some rhizosphere microbes, such as plant growth-promoting rhizobacteria (PGPR), can alleviate plant drought stress and improve water use efficiency and productivity under drought conditions. The aims of this study are: 1) isolation and characterization of PGPRs from the rhizosphe...

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Bibliographic Details
Published in:Plant and soil 2017-10, Vol.419 (1/2), p.523-539
Main Authors: Huang, Xing-Feng, Zhou, Dongmei, Lapsansky, Erin R., Reardon, Kenneth F., Guo, Jianhua, Andales, Marie J., Vivanco, Jorge M., Manter, Daniel K.
Format: Article
Language:English
Subjects:
Antioxidants
Arabidopsis
Arabidopsis thaliana
Bacteria
Biomedical and Life Sciences
Burkholderia
Carboxylic acids
Corn
Drought
Drought resistance
Droughts
Ecology
Evapotranspiration
Health aspects
Inoculation
Life Sciences
Malondialdehyde
Moisture content
Physiological responses
Physiology
Plant growth
Plant Physiology
Plant Sciences
Proline
Regular Article
Rhizosphere
Roots
Soil erosion
Soil Science & Conservation
Soil water
Stress
Stresses
Water loss
Water use
Water use efficiency
Zea mays
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Summary:Background and aims Some rhizosphere microbes, such as plant growth-promoting rhizobacteria (PGPR), can alleviate plant drought stress and improve water use efficiency and productivity under drought conditions. The aims of this study are: 1) isolation and characterization of PGPRs from the rhizosphere of Arabidopsis plants that could improve drought tolerance of Arabidopsis and maize; 2) studying the potential mechanisms of improved plant drought tolerance by the isolated bacteria. Methods In this study, bacteria isolates were isolated from the rhizosphere of Arabidopsis plants subjected to water limitation. Subsequently, the isolates were cultured and screened for their ability to improve drought tolerance of Arabidopsis. Potential mechanisms of improved plant drought tolerance by these bacteria including bacterial exopolysaccharide and 1-aminocyclopropane-1-carboxylic acid deaminase production, plant root system architecture modification, and plant physiological responses were also explored. Results Two bacterial isolates that conferred the greatest drought tolerance to Arabidopsis were further characterized. Both bacteria exhibit 1-aminocyclopropane-1-carboxylic acid deaminase activity, which can promote drought tolerance by decreasing plant ethylene levels. In vitro study showed that both Mitsuaria sp. ADR17 and Burkholderia sp. ADR10 altered the root structure system of Arabidopsis. Moreover, inoculation of Zea mays L. with either strain reduced evapotranspiration (i.e., soil water loss), and changed the plant proline and malondialdehyde levels, antioxidant enzymes activity, and phytohormone contents under drought stress. Conclusions These results indicate that both strains interact with plants in ways that allow them to alter root system architecture, plant physiological responses and phytohormone levels under drought conditions to alleviate stress and improve plant survival. The results also indicated that each individual isolate has a separate pleiotropic effect.
ISSN:0032-079X
1573-5036
DOI:10.1007/s11104-017-3360-4