Isolation of Salt Stress Tolerance Genes from Roots of Mangrove Plant, Rhizophora stylosa Griff., Using PCR-Based Suppression Subtractive Hybridization

Salinity is a major abiotic stress that seriously limits plant growth and crop productivity. The halophytic Rhizophora stylosa is useful for the study of the molecular mechanisms behind salinity tolerance in mangrove trees. To isolate anti-salt stress genes from mangrove plants, a cDNA library of R...

Full description

Saved in:
Bibliographic Details
Published in:Plant molecular biology reporter 2011-09, Vol.29 (3), p.533-543
Main Authors: Basyuni, Mohammad, Kinjo, Yuji, Baba, Shigeyuki, Shinzato, Naoya, Iwasaki, Hironori, Siregar, Edy B. M., Oku, Hirosuke
Format: Article
Language:English
Subjects:
Abiotic stress
Bioinformatics
Biomedical and Life Sciences
Biosynthesis
Crop production
Exposure
Expressed sequence tags
Fresh water
Freshwater environments
Gene expression
Genes
Germination
Hybridization
Life Sciences
Mangrove trees
Metabolism
Metabolomics
Molecular modelling
Plant Breeding/Biotechnology
Plant growth
Plant Sciences
Plants (botany)
Polymerase chain reaction
Protein biosynthesis
Protein synthesis
Protein turnover
Proteomics
Rhizophora
Roots
Salinity
Salinity effects
Salinity tolerance
Salt tolerance
Salts
Seedlings
Stress
Stresses
Tags
Transcription factors
Trees
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Salinity is a major abiotic stress that seriously limits plant growth and crop productivity. The halophytic Rhizophora stylosa is useful for the study of the molecular mechanisms behind salinity tolerance in mangrove trees. To isolate anti-salt stress genes from mangrove plants, a cDNA library of R . stylosa roots was constructed and screened for stress-related genes by polymerase chain reaction (PCR)-based suppressive subtractive hybridization (SSH). The mangrove seedlings were grown for 8 months under two conditions: exposure to water with 3% salt and exposure to fresh water. cDNA of seedlings exposed to water with 3% salt was used as a tester and cDNA for freshwater germination was used as a driver. We isolated and sequenced 240 up-regulated expressed sequence tags (ESTs) from the SSH library. Among these up-regulated ESTs, 48 unique clones were putatively identified and classified into ten functional categories, such as cell rescue and defense, secondary metabolism, protein synthesis, and metabolism. Fifteen genes from different categories were selected and their expression was studied by real-time RT-PCR. Significantly increased expression levels were confirmed for 13 of these 15 transcripts, which suggest that these genes contribute to the salt tolerance of this plant. Among them, two transcription factors and several genes involved in isoprenoid biosynthesis were identified from mangrove trees as salt tolerance genes for the first time. The physiological significance of the increased expression of these genes in the long-term adaptation of mangrove trees to salt stress is discussed.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-010-0257-2