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Genome-wide identification, classification and expression analysis of NAC family of genes in sorghum [Sorghum bicolor (L.) Moench]
NAC transcription factors are involved in many biological processes via regulation of downstream target gene expression and play essential roles in regulation of plant growth and improving plant tolerance to abiotic stress. NAC transcription factors have been studied in various species, but little i...
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Published in: | Plant growth regulation 2017-11, Vol.83 (2), p.301-312 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | NAC transcription factors are involved in many biological processes via regulation of downstream target gene expression and play essential roles in regulation of plant growth and improving plant tolerance to abiotic stress. NAC transcription factors have been studied in various species, but little information is available regarding these factors in sorghum. Genome-wide investigation of potentially abiotic stress related sorghum NAC-type genes was performed. A total of 145 non-redundant
NAC
genes (
SbNAC1
–
SbNAC145
) were identified in the sorghum genome. These genes were distributed unevenly across the 10 chromosomes, and were divided into 16 groups based on sequence similarity. Gene structure analysis indicated that most
SbNAC
genes contained three exons and two introns, and had ten putative conserved motifs. Phylogenetic analysis indicated that the
SbNAC
genes with similar motif distributions were clustered into the same branch. Seven
SbNAC
genes, which were grouped into the stress-related subgroup, were isolated and have been confirmed to have transcriptional activity in yeast.
SbNAC
genes showed differential expression patterns over time in response to dehydration, salinity, cold, and phytohormone abscisic acid stress treatments, thus suggesting essential roles in plant responses to abiotic stress. In the germination stage,
SbNAC56
overexpression transgenic lines exhibited significantly enhanced hypersensitivities to ABA, NaCl and
d
-Mannitol. This may infer that
SbNAC56
may play essential roles in plants response to abiotic stresses in ABA dependent signaling pathway. Here, we present a comprehensive overview of the
SbNAC
genes and provide a foundation for future functional research regarding their biological roles in sorghum stress tolerance, even in the regulation of plant growth. |
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ISSN: | 0167-6903 1573-5087 |
DOI: | 10.1007/s10725-017-0295-y |