Plasmonic cell nanocoating: a new concept for rapid microbial screening

Nanocoating of single microbial cells with gold nanostructures can confer optical, electrical, thermal, and mechanical properties to microorganisms, thus enabling new avenues for their control, study, application, and detection. Cell nanocoating is often performed using layer-by-layer (LbL) depositi...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-11, Vol.409 (27), p.6305-6314
Main Authors: Xu, Ke, Bui, Minh-Phuong N., Fang, Aiqin, Abbas, Abdennour
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bacteria
Biochemistry
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chitin
Chitin - chemistry
Cytochemistry
Disulfides - chemistry
E coli
Electrostatic properties
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Fluorescence
Fluorescent Dyes - chemistry
Food Science
Fungi
Gold
Gold - chemistry
Humans
Laboratory Medicine
Limit of Detection
Mechanical properties
Metal Nanoparticles - chemistry
Microbiological chemistry
Microorganisms
Monitoring/Environmental Analysis
Nanoparticles
Optical properties
Paper in Forefront
Plasmonics
Proteins
Saccharomyces cerevisiae - isolation & purification
Screening
Spectrometry, Fluorescence - methods
Spectrum Analysis, Raman - methods
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Summary:Nanocoating of single microbial cells with gold nanostructures can confer optical, electrical, thermal, and mechanical properties to microorganisms, thus enabling new avenues for their control, study, application, and detection. Cell nanocoating is often performed using layer-by-layer (LbL) deposition. LbL is time-consuming and relies on nonspecific electrostatic interactions, which limit potential applications for microbial diagnostics. Here, we show that, by taking advantage of surface molecules densely present in the microbial outer layers, cell nanocoating with gold nanoparticles can be achieved within seconds using surface molecules, including disulfide- bond-containing (Dsbc) proteins and chitin. A simple activation of these markers and their subsequent interaction with gold nanoparticles allow specific microbial screening and quantification of bacteria and fungi within 5 and 30 min, respectively. The use of plasmonics and fluorescence as transduction methods offers a limit of detection below 35 cfu mL –1 for E. coli bacteria and 1500 cfu mL –1 for M. circinelloides fungi using a hand-held fluorescent reader. Graphical abstract A new concept for rapid microbial screening by targeting disulfide - bond-containing (Dsbc) proteins and chitin with reducing agents and gold nanoparticles.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-017-0612-3