Recycled Paper Sludge Microbial Community as a Potential Source of Cellulase and Xylanase Enzymes

Waste disposal from paper and pulp industry is of great environmental concern. Sludge from recycled paper mills favors colonization with different microbial species due to its chemical composition. In this environment, the microbiota can express enzymes that hydrolyze celluloses and hemicelluloses,...

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Bibliographic Details
Published in:Waste and biomass valorization 2017-09, Vol.8 (6), p.1907-1917
Main Authors: Heinz, Kássia G. H., Zanoni, Patrícia R. S., Oliveira, Rafael R., Medina-Silva, Renata, Simão, Taiz L. L., Trindade, Fernanda J., Pereira, Leandro M., Tavares, Lorena B. B., Giongo, Adriana
Format: Article
Language:English
Subjects:
Bacteria
Cellulase
Colonization
Communities
Deoxyribonucleic acid
DNA
DNA sequencing
Engineering
Environment
Environmental Engineering/Biotechnology
Environmental perception
Enzymes
Fungi
Industrial Pollution Prevention
Industrial wastes
Microorganisms
Original Paper
Paper mills
Pulp & paper industry
Pulp & paper mills
Recycled materials
Renewable and Green Energy
Sludge
Waste disposal
Waste Management/Waste Technology
Wastewater
Xylanase
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Summary:Waste disposal from paper and pulp industry is of great environmental concern. Sludge from recycled paper mills favors colonization with different microbial species due to its chemical composition. In this environment, the microbiota can express enzymes that hydrolyze celluloses and hemicelluloses, such as cellulases, β-glucosidases, and xylanases. Understanding microbiota living in such disposal may give directions to what kind of enzymes or bioproducts may be possible to obtain from this microorganism and how to deal with the waste during the process. We have accessed microbial community associated with two steps in the recycled paper sludge generation by high-throughput DNA sequencing: fibrous residue, and wastewater sludge. Bacteroidetes, Proteobacteria and Firmicutes phyla represented more than 96% of the total. From the eukaryote perspective, Vorticella was the most frequent in fibrous residue samples while Acanthamoeba was the most common on wastewater sludge samples. Among the isolates cellulase activity was detectable only from fungi isolates. Half of the bacterial isolates presented xylanase activity, and most of them in high level of activity. Both a metabarcoding and culturing approaches are valuable tools for enzymatic screening in industrial waste disposal.
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-016-9792-x