Reproducibility of the RAPD marker and its efficiency in coffee tree genotype grouping analysis

The genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primerswere used to evaluate the reproducibilityand the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation...

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Bibliographic Details
Published in:Crop breeding and applied biotechnology 2002-03, Vol.2 (1), p.121-129
Main Authors: Teixeira-Cabral, T.A., Sakiyama, N.S., Zambolim, L., Pereira, A.A., Barros, E.G., Sakiyama, C.C.H.
Format: Article
Language:English
Subjects:
Coffea arabica
Coffee
Composition
Cultivars
Estimation
Genetic analysis
Genetic distance
Genetic diversity
Genotypes
Markers
Plant breeding
Polymerase chain reaction
Random amplified polymorphic DNA
Reproducibility
Subgroups
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Summary:The genetic diversity of Coffea arabic L. cultivars is relatively narrow and its assessment and increase is important for breeding. Fifty two arbitrary primerswere used to evaluate the reproducibilityand the influence of the number of RAPD (Random Amplified Polymorphic DNA) markers on the estimation of genetic distancesamong 40 genotypes of Coffea spp. The average number of polymorphic bands was 6.69 per primer among all genotypes, and 1.27 among arabica coffee genotypes. RAPD markers were efficient in estimating the genetic distances among the genotypes. The increase in RAPD loci number during grouping analysis did not affect the major groups’ composition; however, it affected the composition of subgroups. Marker reproducibility was 76.88% and replicated data was recommended for distinguishing genotypes with the same genetic background.
ISSN:1984-7033
1518-7853
1984-7033
DOI:10.12702/1984-7033.v02n01a16