Characterization of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. with RAPD markers

Eighteen clones of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. were characterized with RAPD markers. The genetic distances were estimated and the genealogical origin of the clones compared to data of marker- based clusters. Thirty-five primers identified 158 polymorphic loci o...

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Bibliographic Details
Published in:Crop breeding and applied biotechnology 2004-03, Vol.4 (1), p.68-73
Main Authors: Teixeira-Cabral, T.A., Sakiyama, N.S., Zambolim, L., Pereira, A.A., Barros, E.G., Silva, D.G.
Format: Article
Language:English
Subjects:
Cloning
Coffea arabica
Coffee
Genetic distance
Hemileia vastatrix
Markers
Random amplified polymorphic DNA
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Summary:Eighteen clones of differential coffee tree hosts for Hemileia vastatrix Berk. et Br. were characterized with RAPD markers. The genetic distances were estimated and the genealogical origin of the clones compared to data of marker- based clusters. Thirty-five primers identified 158 polymorphic loci of RAPD markers. The cluster based on the matrix of genetic dissimilarity values was compatible with information on the genealogical origin cited in literature. Specific markers for a number of clones were identified, and a combination of 12 RAPD markers allowed the characterization of the studied clones.
ISSN:1984-7033
1518-7853
1984-7033
DOI:10.12702/1984-7033.v04n01a11