Inhibitory effects of Asterina pectinifera extracts on melanin biosynthesis through tyrosinase activity

The control of melanogenesis is an important strategy in the treatment of abnormal skin pigmentation for cosmetic purposes. The aim of the present study was to investigate the anti-melanogenic effect of Asterina pectinifera (A. pectinifera) extracts by cell-free mushroom tyrosinase assay, cellular t...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular medicine 2013-01, Vol.31 (1), p.205-212
Main Authors: JEONG, MIN-HO, YANG, KWANG-MO, KIM, JUNG-KI, NAM, BYUNG-HYOUK, KIM, GI-YONG, LEE, SANG-WHA, SEO, SU-YEONG, JO, WOL-SOON
Format: Article
Language:English
Subjects:
Asterina pectinifera
Biosynthesis
Cosmetics
Cytotoxicity
dopachrome tautomerase
Enzymes
Gene expression
hyperpigmentation
melanogenesis
Physiology
Proteins
Shellfish
Skin
Studies
tyrosinase
tyrosinase-related protein-1
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The control of melanogenesis is an important strategy in the treatment of abnormal skin pigmentation for cosmetic purposes. The aim of the present study was to investigate the anti-melanogenic effect of Asterina pectinifera (A. pectinifera) extracts by cell-free mushroom tyrosinase assay, cellular tyrosinase assay, melanin content assay and the analysis of related protein expression in melan-a cells. A. pectinifera was extracted with 80% methanol (80-MAP) and further fractionated with hexane (He-AP) and ethyl acetate (EA-AP). In addition, the enzyme extract (En-AP) of A. pectinifera, to which protease was added, was processed. EA-AP and En-AP among A. pectinifera extracts showed strong inhibitory activity against the cell-free mushroom tyrosinase activity. EA-AP and En-AP induced significant inhibition of melanin production and cellular tyrosinase activity. In the action of EA-AP and En-AP on melanogenesis, they reduced the expression of melanogenic genes and proteins including tyrosinase, tyrosinase-related protein-1 (TRP-1) and dopachrome tautomerase (Dct). These results showed that EA-AP and En-AP inhibited melanogenesis by reducing tyrosinase activity and melanin production via subsequent downregulation of tyrosinase-related proteins. The overall results suggest that EA-AP and En-AP among A. pectinifera extracts may be promising candidates for the treatment of hyperpigmentation disorder and useful for self-tanning cosmetic products.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2012.1181