The impact of space environment on gene expression in Arabidopsis thaliana seedlings

One of the important questions in space biology is the mechanisms underlying plant responses to an outer space environment,i.e.,how gene expression is altered in space.In this study,the transcriptome of Arabidopsis thaliana seedlings was analyzed as a part of Germany SIMBOX（science in microgravity b...

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Bibliographic Details
Published in:Science China. Technological sciences 2017-06, Vol.60 (6), p.902-910
Main Authors: Li, HuaSheng, Lu, JinYing, Zhao, Hui, Sun, Qiao, Yu, FuTong, Pan, Yi, Chen, Yu, Su, Liang, Liu, Min
Format: Article
Language:English
Subjects:
Aerospace environments
Biology
Centrifugal force
Chips
Damage
Deoxyribonucleic acid
DNA
DNA损伤修复
Engineering
Environmental impact
Gene expression
Genes
Ground based control
Knowledge representation
Metabolism
Microgravity
Plants (botany)
Repair
Space flight
Stresses
基因差异表达
基因表达
外部空间环境
差异表达基因
幼苗
拟南芥
航天实验
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Summary:One of the important questions in space biology is the mechanisms underlying plant responses to an outer space environment,i.e.,how gene expression is altered in space.In this study,the transcriptome of Arabidopsis thaliana seedlings was analyzed as a part of Germany SIMBOX（science in microgravity box）spaceflight experiment on Shenzhou 8 spacecraft.This experiment involved the following treatments：spaceflight with microgravity（Fμg）,spaceflight with 1g centrifugal force（F 1g）,and ground 1g control（G 1g）.Gene chips were used to screen gene expression differences in Arabidopsis thaliana seedlings among these treatments.Microarray analysis revealed that 621 genes were differentially expressed in samples Fμg vs.G 1g,249 genes in samples F 1g vs.G 1g,and 368 genes in samples Fμg vs.F 1g.Gene ontology analysis indicated that the genes were involved in metabolism of stress response,gravitropic response,and DNA damage and repair,suggesting that plants adjust these metabolic pathways to space environmental stress,microgravity,and radiation.
ISSN:1674-7321
1869-1900
DOI:10.1007/s11431-016-0232-7