Design and development of hyaluronan-functionalized polybenzofulvene nanoparticles as CD44 receptor mediated drug delivery system

A tri-component polymer brush (TCPB ) , composed of a polybenzofulvene copolymer bearing low molecular weight hyaluronic acid (HA) on the surface of its cylindrical brush-like backbone and oligo-PEG fractions, was employed in the preparation of 350 nm nanostructured drug delivery systems capable of...

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Published in:Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2017-06, Vol.19 (6), p.1, Article 197
Main Authors: Licciardi, Mariano, Scialabba, Cinzia, Giammona, Gaetano, Paolino, Marco, Razzano, Vincenzo, Grisci, Giorgio, Giuliani, Germano, Makovec, Francesco, Cappelli, Andrea
Format: Article
Language:English
Subjects:
Adenocarcinoma
Biotechnology
Breast
Brushes
Cancer
CD44 antigen
Characterization and Evaluation of Materials
Chemistry and Materials Science
Colon
Colon cancer
Cytology
Doxorubicin
Drug delivery
Drug delivery systems
Drug development
Hyaluronic acid
In vitro methods and tests
Inorganic Chemistry
Lasers
Materials Science
Molecular weight
Nanoparticles
Nanostructure
Nanotechnology
Optical Devices
Optics
Photonics
Physical Chemistry
Polyethylene glycol
Research Paper
Staining
Transdermal medication
Zeta potential
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Summary:A tri-component polymer brush (TCPB ) , composed of a polybenzofulvene copolymer bearing low molecular weight hyaluronic acid (HA) on the surface of its cylindrical brush-like backbone and oligo-PEG fractions, was employed in the preparation of 350 nm nanostructured drug delivery systems capable of delivering the anticancer drug doxorubicin. The obtained drug delivery systems were characterized on the basis of drug loading and release, dimensions and zeta potential, morphology and in vitro cell activity, and uptake on three different human cell lines, namely the bronchial epithelial 16HBE, the breast adenocarcinoma MCF-7, and the colon cancer HCT116 cells. Finally, the ability of doxorubicin-loaded TCPB nanoparticles (DOXO-TCPB) to be internalized into cancer cells by CD44 receptor mediated uptake was assessed by means of uptake studies in HCT cells. These data were supported by anti-CD44-FITC staining assay. The proposed TCPB nanostructured drug delivery systems have many potential applications in nanomedicine, including cancer targeted drug delivery. Graphical abstract TCPB forms nanostructured carrier for doxorubicin able to be internalized into cancer cells by CD44 mediated targeting
ISSN:1388-0764
1572-896X
DOI:10.1007/s11051-017-3881-z