Biocatalytic Self‐Assembly Cascades

The properties of supramolecular materials are dictated by both kinetic and thermodynamic aspects, providing opportunities to dynamically regulate morphology and function. Herein, we demonstrate time‐dependent regulation of supramolecular self‐assembly by connected, kinetically competing enzymatic r...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2017-06, Vol.56 (24), p.6828-6832
Main Authors: Sahoo, Jugal Kishore, Pappas, Charalampos G., Sasselli, Ivan Ramos, Abul‐Haija, Yousef M., Ulijn, Rein V.
Format: Article
Language:English
Subjects:
Amidase
biocatalysis
enzyme cascade
Enzymes
pathway selection
peptide amphiphile
Phenylalanine
Phosphates
Self-assembly
supramolecular chemistry
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Summary:The properties of supramolecular materials are dictated by both kinetic and thermodynamic aspects, providing opportunities to dynamically regulate morphology and function. Herein, we demonstrate time‐dependent regulation of supramolecular self‐assembly by connected, kinetically competing enzymatic reactions. Starting from Fmoc‐tyrosine phosphate and phenylalanine amide in the presence of an amidase and phosphatase, four distinct self‐assembling molecules may be formed which each give rise to distinct morphologies (spheres, fibers, tubes/tapes and sheets). By varying the sequence or ratio in which the enzymes are added to mixtures of precursors, these structures can be (transiently) accessed and interconverted. The approach provides insights into dynamic self‐assembly using competing pathways that may aid the design of soft nanostructures with tunable dynamic properties and life times. Finding the right path: Starting from Fmoc‐tyrosine phosphate and phenylalanine amide in the presence of an amidase (orange in scheme) and phosphatase (green), four distinct molecules are formed which each give rise to distinct morphologies: spheres, fibers, tubes/tapes and sheets. By varying the sequence or ratio in which the enzymes are added to mixtures of precursors, these structures can be accessed and interconverted in a controlled manner.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201701870