A pH-Activatable Fluorescent Aptamer Probe for Imaging of Target Cancer Cells

Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, w...

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Bibliographic Details
Published in:Wuhan University journal of natural sciences 2017-06, Vol.22 (3), p.247-251
Main Authors: Zhou, Yan, Fu, Boshi, Xiao, Heng
Format: Article
Language:English
Subjects:
Aptamers
Benign
Biology and Pharmacy
Biomarkers
Biomedical and Life Sciences
Breast cancer
Cancer
Cell surface
Computer Science
Fluorescence
Formaldehyde
Hydrogen ions
Life Sciences
Materials Science
nucleolin
pH effects
Selectivity
Target recognition
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Summary:Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction. The spectral characteristics of fluorophore naphthalimide has a good pH dependence. By this way, nucleolin-overexpressed cell could be discriminated from normal cell. Further, this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded（FFPE） tissue specimens. It is considered that our method has the potential to be applied in medical detection.
ISSN:1007-1202
1993-4998
DOI:10.1007/s11859-017-1242-5