Aqueous humour concentrations of TGF-[beta], PLGF and FGF-1 and total retinal blood flow in patients with early non-proliferative diabetic retinopathy

Purpose To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non-proliferative diabetic retinopathy (NPDR). Methods A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the st...

Full description

Saved in:
Bibliographic Details
Published in:Acta ophthalmologica (Oxford, England) England), 2017-05, Vol.95 (3), p.e206
Main Authors: Khuu, Lee-Anne, Tayyari, Faryan, Sivak, Jeremy M, Flanagan, John G, Singer, Shaun, Brent, Michael H, Huang, David, Tan, Ou, Hudson, Christopher
Format: Article
Language:English
Subjects:
Ophthalmology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose To correlate angiogenic cytokines in the aqueous humour with total retinal blood flow in subjects with type 2 diabetes with non-proliferative diabetic retinopathy (NPDR). Methods A total of 17 controls and 16 NPDR patients were recruited into the study. Aqueous humour was collected at the start of cataract surgery to assess the concentration of 14 angiogenic cytokines. Aqueous humour was analysed using the suspension array method. Six images were acquired to assess total retinal blood flow (TRBF) using the prototype RTVue(TM) Doppler Fourier domain optical coherence tomography (Doppler FD-OCT) (Optovue, Inc., Fremont, CA) using a double circular scan protocol, 1 month postsurgery. At the same visit, forearm blood was collected to determine glycosylated haemoglobin (A1c). Results Transforming growth factor beta (TGF-[beta]1, TGF-[beta]2) and PLGF were increased while FGF-1 was reduced in NPDR compared to controls (Bonferroni corrected, p < 0.003 for all). Total retinal blood flow (TRBF) was significantly reduced in the NPDR group compared to controls (33.1 ± 9.9 versus 43.3 ± 5.3 µl/min, p = 0.002). Aqueous FGF-1 significantly correlated with TRBF in the NPDR group (r = 0.71, p = 0.01; r2 = 0.51). In a multiple regression analysis, A1c was found to be a significant predictor of aqueous TGF-[beta]1 and FGF-1 (p = 0.018 and p = 0.020, respectively). Conclusion Aqueous angiogenic cytokines (TGF-[beta]1, TGF-[beta]2 and PLGF) were elevated in conjunction with a reduction in TRBF in patients with NPDR compared to controls. Non-invasive measurement of TRBF may be useful for predicting aqueous FGF-1 levels and severity of vasculopathy in DR.
ISSN:1755-375X
1755-3768
DOI:10.1111/aos.13230