Gene expression analysis of the withanolide biosynthetic pathway in hairy root cultures of Withania somnifera elicited with methyl jasmonate and the fungus Piriformospora indica
Withania somnifera , also known as Indian ginseng is known to contain valuable bioactive compounds, called withanolides that structurally resemble ginsenosides of Panax ginseng . These compounds provide the basis of pharmacological relevance in traditional systems of medicine. In the present study,...
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Published in: | Symbiosis (Philadelphia, Pa.) Pa.), 2017-02, Vol.71 (2), p.143-154 |
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Main Authors: | , , , , |
Format: | Article |
Language: | eng |
Subjects: | |
Online Access: | Get full text |
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Summary: | Withania somnifera
, also known as Indian ginseng is known to contain valuable bioactive compounds, called withanolides that structurally resemble ginsenosides of
Panax ginseng
. These compounds provide the basis of pharmacological relevance in traditional systems of medicine. In the present study, 150 hairy root lines of
W. somnifera
were induced of which nine fast growing lines were analysed for their growth and withanolide content. Hairy root line W9 was selected due to its high specific growth rate (0.196 ± 0.005 d
−1
) and high withanolide content. The response to different concentrations of elicitors (methyl jasmonate and
P. indica
cell homogenate) and various exposure durations was assessed in the W9 hairy root line. The withanolide content as well as the pattern of gene expression from MVA, MEP and sterol pathway, was evaluated using qPCR. Though gene expression and withanolide content were found to be elevated in almost all MeJ and CHP treatments, the exposure of hairy roots to 15 μM MeJ for 4 h gave the maximum withanolide yield. The results suggest that the elicitation potential of methyl jasmonate was higher than that of
P. indica
cell homogenate for increasing withanolide levels in hairy roots of
W. somnifera
. |
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ISSN: | 0334-5114 1878-7665 |