Structural Insights into the Bovine Casein Micelle: Small Angle X-Ray Scattering Studies and Correlations with Spectroscopy

In the processing of milk into dairy products, the structure, stability, and dynamics of the casein micelle play definitive roles. These colloidal casein complexes are the result of an ensemble of multiple competing equilibria, arising from 1°, 2°, 3°, and 4° interactions of the individual caseins w...

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Bibliographic Details
Published in:Journal of dairy science 1990-12, Vol.73 (12), p.3592-3601
Main Authors: Farrell, H.M., Pessen, H., Brown, E.M., Kumosinski, T.F.
Format: Article
Language:English
Subjects:
ANALYTICAL METHODS
Biological and medical sciences
CASEIN
CASEINA
CASEINE
DENSIDAD
DENSITE
DENSITY
Food industries
Fundamental and applied biological sciences. Psychology
Milk and cheese industries. Ice creams
MOLECULAR CONFORMATION
MOLECULAR WEIGHT
PESO MOLECULAR
POIDS MOLECULAIRE
protein structure
small angle x-ray scattering
STRUCTURE
SUBMICELLES
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
X RAY DIFFRACTION
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Summary:In the processing of milk into dairy products, the structure, stability, and dynamics of the casein micelle play definitive roles. These colloidal casein complexes are the result of an ensemble of multiple competing equilibria, arising from 1°, 2°, 3°, and 4° interactions of the individual caseins with themselves and with milk salts. Our laboratory has studied the fundamental principles of casein structure by application of modern methods of protein chemistry. The dynamics and quaternary structure of this interacting system have been examined using small-angle x-ray scattering. These data are now correlated with information obtained from [2H] nuclear magnetic resonance relaxation techniques, and with insights into the secondary structures of the caseins gained by Raman and Fourier-transform infrared spectroscopy. Results are interpreted in terms of a model where hydrophobically stabilized inhomogeneous submicelles are incorporated into the colloidal micelle while retaining most of their structural and dynamic properties.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(90)79062-5