A new approach for kLa determination by gassing-out method in pneumatic bioreactors

BACKGROUND Determination of the volumetric oxygen transfer coefficient (kLa) is very important for bioreactor design and process analysis. Among all the methods available, the gassing‐out technique is one of the simplest and most widely used for this task. However, this method is very sensitive to t...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2016-12, Vol.91 (12), p.3061-3069
Main Authors: Cerri, Marcel Otavio, Nordi Esperança, Mateus, Colli Badino, Alberto, Perencin de Arruda Ribeiro, Marcelo
Format: Article
Language:English
Subjects:
bioreactors
estimation of kLa
gassing-out method
oxygen transfer
probe delay response
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Summary:BACKGROUND Determination of the volumetric oxygen transfer coefficient (kLa) is very important for bioreactor design and process analysis. Among all the methods available, the gassing‐out technique is one of the simplest and most widely used for this task. However, this method is very sensitive to the probe dynamics (due to delay effects), which can lead to inaccuracy in the kLa determination. This is especially pronounced for airlift bioreactors, where the circulation time affects the initial dynamics of the assay (a transient effect). In the present work, a new method for kLa determination is proposed. RESULTS Values of kLa and the electrode response time (τe) were determined experimentally, employing the traditional gassing‐out method and the method proposed here. The latter was shown to be reliable, easier to apply, and suitable for the simultaneous determination of kLa and electrode sensitivity (ke = 1/τe), without any need for additional assays. CONCLUSION The new method overcomes the effects of probe delay on kLa determination, making the procedure faster and simpler. © 2016 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.4937