Dual-Reactable Fluorescent Probes for Highly Selective and Sensitive Detection of Biological H2S

Hydrogen sulfide (H2S) is an important endogenous signaling molecule with a variety of biological functions. Development of fluorescent probes for highly selective and sensitive detection of H2S is necessary. We show here that dual‐reactable fluorescent H2S probes could react with higher selectivity...

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Bibliographic Details
Published in:Chemistry, an Asian journal an Asian journal, 2016-05, Vol.11 (9), p.1376-1381
Main Authors: Wei, Chao, Wang, Runyu, Zhang, Changyu, Xu, Guoce, Li, Yanyan, Zhang, Qiang-Zhe, Li, Lu-Yuan, Yi, Long, Xi, Zhen
Format: Article
Language:English
Subjects:
Chemistry
dual-quenching
dual-reactable
fluorescent imaging
hydrogen sulfide
imaging agents
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Summary:Hydrogen sulfide (H2S) is an important endogenous signaling molecule with a variety of biological functions. Development of fluorescent probes for highly selective and sensitive detection of H2S is necessary. We show here that dual‐reactable fluorescent H2S probes could react with higher selectivity than single‐reactable probes. One of the dual‐reactable probes gives more than 4000‐fold turn‐on response when reacting with H2S, the largest response among fluorescent H2S probes reported thus far. In addition, the probe could be used for high‐throughput enzymatic assays and for the detection of Cys‐induced H2S in cells and in zebrafish. These dual‐reactable probes hold potential for highly selective and sensitive detection of H2S in biological systems. Two heads are better than one: Fluorescent probes with two types of reactive heads (R1 and R2) for H2S can lead to a higher selectivity than that of single‐reactable probes (See Figure). The probe was used for highly selective and sensitive detection of biological H2S.
ISSN:1861-4728
1861-471X
DOI:10.1002/asia.201600262