The effect of chromophore charge on the incorporation efficiency of fluorescence-labeled nucleotides catalyzed by Taq DNA polymerase in matrix synthesis

The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP derivatives that contain different fluorescent labels, i.e., electroneutral, positively charged, and negatively charged Cy5 analogs. dUTP labele...

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Bibliographic Details
Published in:Biophysics (Oxford) 2015-11, Vol.60 (6), p.1013-1015
Main Authors: Shershov, V. E., Kuznetsova, V. E., Lysov, Yu. P., Guseinov, T. O., Barsky, V. E., Spitsyn, M. A., Zasedateleva, O. A., Vasiliskov, V. A., Surzhikov, S. A., Zasedatelev, A. S., Chudinov, A. V.
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biophysics
Letters to the Editor
Physics
Physics and Astronomy
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Summary:The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP derivatives that contain different fluorescent labels, i.e., electroneutral, positively charged, and negatively charged Cy5 analogs. dUTP labeled with an electroneutral Cy5 analog was shown to be most efficiently incorporated into DNA when Tag polymerase was used in PCR.
ISSN:0006-3509
1555-6654
DOI:10.1134/S0006350915060238