AlCl3 induces lymphocyte apoptosis in rats through the mitochondria-caspase dependent pathway

ABSTRACT To investigate apoptosis mechanisms in lymphocytes induced by aluminum trichloride (AlCl3) through the mitochondria–caspase dependent pathway, the spleen lymphocytes of rats were cultured with RPMI‐1640 medium and exposed to AlCl3·6H2O in the final concentrations of 0 (control group, CG), 0...

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Published in:Environmental toxicology 2016-04, Vol.31 (4), p.385-394
Main Authors: Li, Miao, Song, Miao, Ren, Li-Min, Xiu, Chun-Yu, Liu, Jian-Yu, Zhu, Yan-zhu, Li, Yan-Fei
Format: Article
Language:English
Subjects:
aluminum
Aluminum Compounds - toxicity
Animals
Apoptosis
Apoptosis - drug effects
Apoptosis - physiology
bcl-2-Associated X Protein - genetics
caspase
Caspase 3 - genetics
Caspase 3 - metabolism
Caspase 9 - genetics
Caspase 9 - metabolism
Cells, Cultured
Chlorides - toxicity
Cytochromes c - metabolism
Lymphocytes
Lymphocytes - drug effects
Lymphocytes - metabolism
Membrane Potential, Mitochondrial - drug effects
Mitochondria
Mitochondria - metabolism
Protein expression
Proto-Oncogene Proteins c-bcl-2 - genetics
rat spleen lymphocytes
Rats, Wistar
RNA, Messenger - metabolism
Rodents
Signal Transduction - drug effects
Spleen - cytology
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Summary:ABSTRACT To investigate apoptosis mechanisms in lymphocytes induced by aluminum trichloride (AlCl3) through the mitochondria–caspase dependent pathway, the spleen lymphocytes of rats were cultured with RPMI‐1640 medium and exposed to AlCl3·6H2O in the final concentrations of 0 (control group, CG), 0.3 (low‐dose group, LG), 0.6 (mid‐dose group, MG), and 1.2 (high‐dose group, HG) mmol·L−1 for 24 h, respectively. Mitochondrial transmembrane potential (ΔΨm), cytochrome C (Cyt C) protein expression in cytoplasm, Caspase‐3 and Caspase‐9 activity, Bcl‐2, Bax, Caspase‐3 and Caspase‐9 mRNA expressions, DNA ladder and lymphocytes apoptosis index were detected. The results showed that Cyt C protein expression in cytoplasm, Caspase‐3 and Caspase‐9 activity, Bcl‐2, Bax, Caspase‐3 and Caspase‐9 mRNA expressions, the ratio of Bcl‐2 and Bax mRNA expression, lymphocytes apoptosis index increased, while ΔΨm decreased in the AlCl3‐treated groups compared with those in CG. The results indicate that AlCl3 induces lymphocyte apoptosis in rats through the mitochondria–caspase dependent pathway. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 385–394, 2016.
ISSN:1520-4081
1522-7278
DOI:10.1002/tox.22051