Direct Biofluid Analysis Using Hydrophobic Paper Spray Mass Spectrometry

Ambient electrostatic paper spray ionization from a hydrophobic paper occurs when a DC potential is applied to the dry paper triangle. Online liquid/liquid extraction of small organic compounds from a drop of biological fluid present on the dry hydrophobic paper is achieved with an organic spray sol...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-02, Vol.88 (3), p.1878-1884
Main Authors: Damon, Deidre E, Davis, Kathryn M, Moreira, Camila R, Capone, Patricia, Cruttenden, Riley, Badu-Tawiah, Abraham K
Format: Article
Language:English
Subjects:
Analytical chemistry
Biomarkers
Body Fluids - chemistry
Electrostatics
Humans
Hydrophobic and Hydrophilic Interactions
Liver diseases
Mass spectrometry
Organic chemicals
Point-of-Care Testing
Spectrometry, Mass, Electrospray Ionization - methods
Street Drugs - blood
Street Drugs - urine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ambient electrostatic paper spray ionization from a hydrophobic paper occurs when a DC potential is applied to the dry paper triangle. Online liquid/liquid extraction of small organic compounds from a drop of biological fluid present on the dry hydrophobic paper is achieved with an organic spray solvent in under 1 min and utilizes in situ electrostatic-spray ionization for more efficient detection of extracted molecules. Direct analysis of small volumes of biofluids with no sample pretreatment is possible, which is applicable in point-of-care analyses. High sensitivity and quantitative accuracy was achieved for the direct analysis of illicit drugs in 4 μL of raw blood, serum, and whole urine. The study was extended to monitor the activity of alanine transaminase enzyme, a key biomarker for the detection of liver injury in patients (with HIV and tuberculosis) who typically take several medications at once.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b04278