MicroRNA-204-5p inhibits gastric cancer cell proliferation by downregulating USP47 and RAB22A

MicroRNAs (miRNAs) are a type of small noncoding RNAs that are strongly implicated in carcinogenesis. However, the potential diagnostic, prognostic and therapeutic roles of the majority of miRNAs in the pathological processes of tumorigenesis remain largely unknown. Our and others’ data revealed tha...

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Bibliographic Details
Published in:Medical oncology (Northwood, London, England) London, England), 2015, Vol.32 (1), p.331, Article 331
Main Authors: Zhang, Binbin, Yin, Yuan, Hu, Yaling, Zhang, Jiwei, Bian, Zehua, Song, Mingxu, Hua, Dong, Huang, Zhaohui
Format: Article
Language:English
Subjects:
Blotting, Western
Cell Proliferation
Down-Regulation
Gene Expression Regulation, Neoplastic - genetics
Hematology
Humans
Immunohistochemistry
Internal Medicine
Medicine
Medicine & Public Health
MicroRNAs - genetics
Oncology
Original Paper
Pathology
rab GTP-Binding Proteins - biosynthesis
rab GTP-Binding Proteins - genetics
Real-Time Polymerase Chain Reaction
RNA, Small Interfering
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Stomach Neoplasms - pathology
Ubiquitin Thiolesterase - biosynthesis
Ubiquitin Thiolesterase - genetics
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Summary:MicroRNAs (miRNAs) are a type of small noncoding RNAs that are strongly implicated in carcinogenesis. However, the potential diagnostic, prognostic and therapeutic roles of the majority of miRNAs in the pathological processes of tumorigenesis remain largely unknown. Our and others’ data revealed that miR-204-5p was significantly downregulated in gastrointestinal tumor tissues compared with adjacent noncancerous tissues. The downregulation of miR-204-5p was confirmed in our gastric cancer (GC) cohort, and we showed that ectopic expression of miR-204-5p inhibited, whereas silencing miR-204-5p expression promoted GC cell proliferation in vitro. Subsequent mechanistic investigations identified that USP47 and RAB22A are direct functional targets of miR-204-5p in GC. Silencing the expression of USP47 and RAB22A using siRNA phenocopied the proliferation-inhibiting function of miR-204-5p in GC cells. Our results uncovered that miR-204-5p acts as a tumor suppressor in GC through inhibiting USP47 and RAB22A.
ISSN:1357-0560
1559-131X
DOI:10.1007/s12032-014-0331-y