High Polymorphism in Est-SSR Loci for Cellulose Synthase and [beta]-Amylase of Sugarcane Varieties (Saccharum spp.) Used by the Industrial Sector for Ethanol Production

High and low polymorphisms in simple sequence repeats of expressed sequence tag (EST-SSR) for specific proteins and enzymes, such as [beta]-amylase, cellulose synthase, xyloglucan endotransglucosylase, fructose 1,6-bisphosphate aldolase, and fructose 1,6-bisphosphatase, were used to illustrate the g...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2015-01, Vol.175 (2), p.965
Main Authors: Augusto, Raphael, Maranho, Rone Charles, Mangolin, Claudete Aparecida, Pires Da Silva Machado, Maria De, Fátima
Format: Article
Language:English
Subjects:
Bioengineering
Enzymes
Ethanol
Polymorphism
Sugarcane
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Summary:High and low polymorphisms in simple sequence repeats of expressed sequence tag (EST-SSR) for specific proteins and enzymes, such as [beta]-amylase, cellulose synthase, xyloglucan endotransglucosylase, fructose 1,6-bisphosphate aldolase, and fructose 1,6-bisphosphatase, were used to illustrate the genetic divergence within and between varieties of sugarcane (Saccharum spp.) and to guide the technological paths to optimize ethanol production from lignocellulose biomass. The varieties RB72454, RB867515, RB92579, and SP813250 on the second stage of cutting, all grown in the state of Paraná (PR), and the varieties RB92579 and SP813250 cultured in the PR state and in Northeastern Brazil, state of Pernambuco (PE), were analyzed using five EST-SSR primers for EstC66, EstC67, EstC68, EstC69, and EstC91 loci. Genetic divergence was evident in the EstC67 and EstC69 loci for [beta]-amylase and cellulose synthase, respectively, among the four sugarcane varieties. An extremely high level of genetic differentiation was also detected in the EstC67 locus from the RB82579 and SP813250 varieties cultured in the PR and PE states. High polymorphism in SSR of the cellulose synthase locus may explain the high variability of substrates used in pretreatment and enzymatic hydrolysis processes, which has been an obstacle to effective industrial adaptations.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-014-1340-1