New structures and composition of cell wall teichoic acids from Nocardiopsis synnemataformans, Nocardiopsishalotolerans, Nocardiopsis composta and Nocardiopsis metallicus: a chemotaxonomic value

The structures of the cell wall teichoic acids (TA) from some species of the genus Nocardiopsis were established by chemical and NMR spectroscopic methods. The cell walls of Nocardiopsis synnemataformans VKM Ac-2518 T and Nocardiopsis halotolerans VKM Ac-2519 T both contain two TA with unique struct...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2014-12, Vol.106 (6), p.1105-1117
Main Authors: Tul’skaya, Elena M., Shashkov, Alexander S., Streshinskaya, Galina M., Potekhina, Natalia V., Evtushenko, Ludmila I.
Format: Article
Language:English
Subjects:
Acids
Biomedical and Life Sciences
Cellular biology
Life Sciences
Medical Microbiology
Microbiology
NMR
Nuclear magnetic resonance
Original Paper
Phosphates
Phylogenetics
Plant Sciences
Soil Science & Conservation
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Summary:The structures of the cell wall teichoic acids (TA) from some species of the genus Nocardiopsis were established by chemical and NMR spectroscopic methods. The cell walls of Nocardiopsis synnemataformans VKM Ac-2518 T and Nocardiopsis halotolerans VKM Ac-2519 T both contain two TA with unique structures—poly(polyol phosphate-glycosylpolyol phosphate)—belonging to the type IV TA. In both organisms, the minor TA have identical structures: poly(glycerol phosphate- N -acetyl-β-galactosaminylglycerol phosphate) with the phosphodiester bond between C-3 of glycerol and C-4 of the amino sugar. This structure is found for the first time. The major TA of N. halotolerans has a hitherto unknown structure: poly(glycerol phosphate- N -acetyl-β-galactosaminylglycerol phosphate), the N -acetyl-β-galactosamine being acetalated with pyruvic acid at positions 4 and 6. The major TA of N. synnemataformans is a poly(glycerol phosphate- N -acetyl-β-galactosaminylglycerol phosphate) with the phosphodiester bond between C-3 of glycerol and C-3 of the amino sugar. The cell walls of Nocardiopsis composta VKM Ac-2520 and N. composta VKM Ac-2521 T contain only one TA, namely 1,3-poly(glycerol phosphate) partially substituted with N -acetyl-α-glucosamine. The cell wall of Nocardiopsis metallicus VKM Ac-2522 T contains two TA. The major TA is 1,5-poly(ribitol phosphate), each ribitol unit carrying a pyruvate ketal group at positions 2 and 4. The structure of the minor TA is the same as that of N. composta . The results presented correlate well with the phylogenetic grouping of strains and confirm the species and strain specific features of cell wall TA in members of the genus Nocardiopsis .
ISSN:0003-6072
1572-9699
DOI:10.1007/s10482-014-0280-7