Immunization with the cysteine proteinaseLdccys1gene fromLeishmania (Leishmania) chagasiand the recombinant Ldccys1 protein elicits protective immune responses in a murine model of visceral leishmaniasis

The geneLdccys1enconding a cysteine proteinase of 30kDa fromLeishmania (Leishmania) chagasi, as well as the recombinant cysteine proteinase rLdccys1, obtained by cloning and expression of theLdccys1gene in the pHIS vector, were used to evaluate their ability to induce immune protective responses in...

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Bibliographic Details
Published in:Vaccine 2008-01, Vol.26 (5), p.677
Main Authors: Ferreira, Josie Haydée L, Gentil, Luciana Girotto, Dias, Suzana Souza, Fedeli, Carlos Eduardo C, Katz, Simone, Barbiéri, Clara Lúcia
Format: Article
Language:English
Subjects:
Bacteriology
Genes
Parasites
Parasitic diseases
Plasmids
Rodents
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Summary:The geneLdccys1enconding a cysteine proteinase of 30kDa fromLeishmania (Leishmania) chagasi, as well as the recombinant cysteine proteinase rLdccys1, obtained by cloning and expression of theLdccys1gene in the pHIS vector, were used to evaluate their ability to induce immune protective responses in BALB/c mice againstL. (L.) chagasiinfection. Mice were immunized subcutaneously with rLdccys1 plus Bacille Calmette Guerin (BCG) orPropionibacterium acnesas adjuvants or intramuscularly with a plasmid carrying theLdccys1gene (Ldccys1/pcDNA3) and CpG ODN as the adjuvant, followed by a booster with rLdccys1 plus CpG ODN. Two weeks after immunization the animals were challenged with 1x107amastigotes ofL. (L.) chagasi. Both immunization protocols induced significant protection againstL. (L.) chagasiinfection as shown by a very low parasite load in the spleen of immunized mice compared to the non-immunized controls. However, DNA immunization was 10-fold more protective than immunization with the recombinant protein. Whereas rLdccys1 induced a significant secretion of IFN-γ and nitric oxide (NO), animals immunized with theLdccys1gene increased the production of IgG2a antibodies, IFN-γ and NO. These results indicated that protection triggered by the two immunization protocols was correlated to a predominant Th1 response.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2007.11.044