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Protective immunity againstIxodes ricinusinduced by a salivary serpin
Iris is a specific elastase inhibitor expressed in the salivary glands of the hard tickIxodes ricinus. It belongs to the superfamily of serpins and interferes with both haemostasis and the immune response of the host. In this study, we first show that Iris is expressed in nymphs but not in the femal...
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Published in: | Vaccine 2007-04, Vol.25 (17), p.3284 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | Iris is a specific elastase inhibitor expressed in the salivary glands of the hard tickIxodes ricinus. It belongs to the superfamily of serpins and interferes with both haemostasis and the immune response of the host. In this study, we first show that Iris is expressed in nymphs but not in the female midgut nor in males. We also show that Iris is present in the saliva. To examine its potency as anti-tick vaccine candidate, we set up three models ofI. ricinusinfestation on immunized animals: nymphs on mice, and adults and nymphs on rabbits. We report the rise of neutralizing antibodies following immunization of rabbits and mice. This comes with a significant protective immunity against ticks in rabbits only, resulting in a 30% mortality rate and a diminution of weight gain in both nymphs and adults and a prolongation of blood feeding time in adults. This is the first report on an anti-tick vaccine trial onI. ricinususing a protein able to interact with both host immunity and haemostasis, as a vaccinating antigen. |
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ISSN: | 0264-410X 1873-2518 |
DOI: | 10.1016/j.vaccine.2007.01.008 |