Fluorescence lifetime imaging microscopy in the medical sciences

The steady improvement in the imaging of cellular processes in living tissue over the last 10–15 years through the use of various fluorophores including organic dyes, fluorescent proteins and quantum dots, has made observing biological events common practice. Advances in imaging and recording techno...

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Bibliographic Details
Published in:Protoplasma 2014-03, Vol.251 (2), p.293-305
Main Authors: Ebrecht, René, Don Paul, Craig, Wouters, Fred S.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cell Biology
Fluorescence Resonance Energy Transfer - methods
Fluorescent Dyes - chemistry
Humans
Life Sciences
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Plant Sciences
Quantum Dots
Special Issue: New/Emerging Techniques in Biological Microscopy
Zoology
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Summary:The steady improvement in the imaging of cellular processes in living tissue over the last 10–15 years through the use of various fluorophores including organic dyes, fluorescent proteins and quantum dots, has made observing biological events common practice. Advances in imaging and recording technology have made it possible to exploit a fluorophore's fluorescence lifetime. The fluorescence lifetime is an intrinsic parameter that is unique for each fluorophore, and that is highly sensitive to its immediate environment and/or the photophysical coupling to other fluorophores by the phenomenon Förster resonance energy transfer (FRET). The fluorescence lifetime has become an important tool in the construction of optical bioassays for various cellular activities and reactions. The measurement of the fluorescence lifetime is possible in two formats; time domain or frequency domain, each with their own advantages. Fluorescence lifetime imaging applications have now progressed to a state where, besides their utility in cell biological research, they can be employed as clinical diagnostic tools. This review highlights the multitude of fluorophores, techniques and clinical applications that make use of fluorescence lifetime imaging microscopy (FLIM).
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-013-0598-4