Mechanisms affecting neutrophil migration capacity in breast cancer patients before and after chemotherapy

Purpose To investigate the mechanisms affecting neutrophil migration capacity in breast cancer patients before and after chemotherapy. Methods Peripheral venous blood was collected at the time of diagnosis and immediately prior to the 4th cycle of an anthracycline-based chemotherapy regimen for pati...

Full description

Saved in:
Bibliographic Details
Published in:Cancer chemotherapy and pharmacology 2014-02, Vol.73 (2), p.317-324
Main Authors: Mendonça, Maria Angélica Oliveira, Souto, Fabrício O., Micheli, Douglas C., Alves-Filho, José Carlos, Cunha, Fernando Q., Murta, Eddie Fernando C., Tavares-Murta, Beatriz M.
Format: Article
Language:English
Subjects:
Actins - blood
Adult
Aged
Antineoplastic agents
Antineoplastic Combined Chemotherapy Protocols - therapeutic use
Biological and medical sciences
Breast cancer
Breast Neoplasms - blood
Breast Neoplasms - drug therapy
Breast Neoplasms - pathology
Cancer Research
Case-Control Studies
Cell Movement - physiology
Cyclophosphamide - administration & dosage
Cytokines - blood
Doxorubicin - administration & dosage
Epirubicin - administration & dosage
Female
Fluorouracil - administration & dosage
Gynecology. Andrology. Obstetrics
Humans
Immune System Diseases - pathology
Interleukin-8 - blood
Leukocyte Disorders - pathology
Mammary gland diseases
Medical sciences
Medicine
Medicine & Public Health
Middle Aged
Multiple tumors. Solid tumors. Tumors in childhood (general aspects)
Neutrophils - metabolism
Neutrophils - pathology
Nitric Oxide - blood
Oncology
Original Article
Pharmacology. Drug treatments
Pharmacology/Toxicology
Receptors, CXCR - blood
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose To investigate the mechanisms affecting neutrophil migration capacity in breast cancer patients before and after chemotherapy. Methods Peripheral venous blood was collected at the time of diagnosis and immediately prior to the 4th cycle of an anthracycline-based chemotherapy regimen for patients diagnosed with different stages of breast cancer ( n  = 30), for experimental assays. Blood samples were also collected from a healthy control group ( n  = 17). Results IL-8 serum concentrations were higher in the patient group than in the control group ( p  = 0.02), and chemotherapy did not further affect this increase. Levels of TNF-α, IL-6, and IL-10 did not differ between controls and patients, or in relation to chemotherapy. Serum levels of nitric oxide (NO) metabolites were elevated following chemotherapy compared to levels detected prior to treatment ( p  = 0.01). When the supernatants of lipopolysaccharide-stimulated mononuclear cells and neutrophils obtained from the patients were assayed for levels of nitrite, these levels were significantly higher and unchanged, respectively, compared with controls. Expression levels of the chemokine receptors, CXCR1 and CXCR2, were significantly reduced in patients compared to controls, and chemotherapy did not further affect these differences. Furthermore, filamentous actin content for IL-8-activated neutrophils was reduced with chemotherapy (median 8.85; range 3.38–13.43) compared to the content detected prior to treatment (median 9.23; range 2.86–22.16) ( p  = 0.001). Conclusion Elevated systemic levels of IL-8 and NO, desensitization to CXCR activation, and reduction in actin polymerization may affect neutrophil motility in patients before and after chemotherapy.
ISSN:0344-5704
1432-0843
DOI:10.1007/s00280-013-2348-x