BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells

DEC1 (BHLHE40/Stra13/Sharp2) and DEC2 (BHLHE41/Sharp1) are basic helix-loop-helix (bHLH) transcription factors that are involved in the regulation of apoptosis, cell proliferation, circadian rhythms and the response to hypoxia. We previously showed the functional effects of DEC1 and DEC2 on apoptosi...

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Bibliographic Details
Published in:Biomedical Research 2012, Vol.33(2), pp.75-82
Main Authors: Wu, Yunyan, Sato, Fuyuki, Bhawal, Ujjal Kumar, Kawamoto, Takeshi, Fujimoto, Katsumi, Noshiro, Mitsuhide, Seino, Hiroko, Morohashi, Satoko, Kato, Yukio, Kijima, Hiroshi
Format: Article
Language:English
Subjects:
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis Regulatory Proteins - genetics
Apoptosis Regulatory Proteins - metabolism
Basic Helix-Loop-Helix Transcription Factors - genetics
Basic Helix-Loop-Helix Transcription Factors - metabolism
Basic Helix-Loop-Helix Transcription Factors - physiology
Bcl-2-Like Protein 11
Caspases - metabolism
Cell Line, Tumor
Cell Nucleus - metabolism
Cell Survival - drug effects
Cisplatin - pharmacology
Gene Expression - drug effects
Humans
Membrane Proteins - genetics
Membrane Proteins - metabolism
Mouth Neoplasms
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases - metabolism
Proto-Oncogene Proteins - genetics
Proto-Oncogene Proteins - metabolism
Tumor Suppressor Proteins - physiology
Up-Regulation - drug effects
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Summary:DEC1 (BHLHE40/Stra13/Sharp2) and DEC2 (BHLHE41/Sharp1) are basic helix-loop-helix (bHLH) transcription factors that are involved in the regulation of apoptosis, cell proliferation, circadian rhythms and the response to hypoxia. We previously showed the functional effects of DEC1 and DEC2 on apoptosis in human breast cancer MCF-7 cells. However, the roles of DEC1 and DEC2 in oral cancer are poorly understood. We examined whether DEC1 and DEC2 are involved in the regulation of apoptosis in human oral cancer HSC-3 and CA9-22 cells. The expression of DEC2 was upregulated by cis-diamminedichloroplatinum (II) (cisplatin: CDDP) treatment in HSC-3 cells, whereas CDDP treatment had little effects on the expression of DEC2 in CA9-22 cells. We showed that DEC2 overexpression inhibits pro-apoptotic factor Bim and inhibits apoptosis induced by CDDP in HSC-3 cells, whereas it had little effects on apoptosis in CA9-22 cells. DEC1 overexpression had little effects on apoptosis induced by CDDP in these cells. We also found that CDDP upregulated the amounts of DEC2 in the nucleus in HSC-3 cells. These results suggest that DEC2 has anti-apoptotic effects on apoptosis induced by CDDP in HSC-3 cells.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.33.75