Inhibitory Effect of Extracts from the Brown Alga, Ecklonia stolonifera, on Enzymes Responsible for Allergic Reactions and Degranulation in RBL-2H3 Cells

The anti-allergic effects of the edible brown alga, Ecklonia stolonifera, are known to affect both degranulation in KU812 cells and anti-inflammatory properties of RAW 264.7 cells. Additional anti-allergic effects of E. stolonifera extracts were therefore investigated by examining the inhibitory eff...

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Bibliographic Details
Published in:FOOD SCIENCE AND TECHNOLOGY RESEARCH 2012, Vol.18(3), pp.467-471
Main Authors: SUGIURA, Yoshimasa, TORII, Takayoshi, TANAKA, Ryusuke, MATSUSHITA, Teruo
Format: Article
Language:eng ; jpn
Subjects:
degranulation
Ecklonia stolonifera
eicosanoid synthesis
inflammation
phlorotannin
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Summary:The anti-allergic effects of the edible brown alga, Ecklonia stolonifera, are known to affect both degranulation in KU812 cells and anti-inflammatory properties of RAW 264.7 cells. Additional anti-allergic effects of E. stolonifera extracts were therefore investigated by examining the inhibitory effects of algal extracts on enzymes associated with eicosanoid synthesis, the inflammation reaction, and anti-degranulation activity in rat basophilic leukemia (RBL) -2H3 cells. A methanol: chloroform (1: 2, v/v) extract of the alga inhibited the enzyme activities and degranulation in stimulated RBL cells in a dose-dependent manner. When phenolic compounds were removed from the M/C extract by treatment with polyvinylpolypyrrolidone resin, the obtained residue did not exhibit the same inhibitory activities. These results imply that the active compounds present in the M/C extract may be phenolic compounds, such as phlorotannins. In addition, the novel anti-allergic effects of E. stolonifera that contributed to inhibiting the enzymatic activities were also clarified.
ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.18.467