Yeast metabolic and signaling genes are required for heat-shock survival and have little overlap with the heat-induced genes

Genome-wide gene-expression studies have shown that hundreds of yeast genes are induced or repressed transiently by changes in temperature; many are annotated to stress response on this basis. To obtain a genome-scale assessment of which genes are functionally important for innate and/or acquired th...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2013-11, Vol.110 (46), p.E4393-E4402
Main Authors: Gibney, Patrick A, Lu, Charles, Caudy, Amy A, Hess, David C, Botstein, David
Format: Article
Language:English
Subjects:
Biological Sciences
Chromatin
DNA Barcoding, Taxonomic
DNA Primers - genetics
Gene Deletion
Gene expression
Gene Expression Profiling - methods
Gene Expression Regulation, Fungal - genetics
Genes
Genetic Markers - genetics
Genetics
Heat
Heat-Shock Response - genetics
High-Throughput Nucleotide Sequencing
Metabolic Networks and Pathways - genetics
Molecular Sequence Annotation
PNAS Plus
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Signal Transduction - genetics
Stress
Systems Biology - methods
Yeast
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Summary:Genome-wide gene-expression studies have shown that hundreds of yeast genes are induced or repressed transiently by changes in temperature; many are annotated to stress response on this basis. To obtain a genome-scale assessment of which genes are functionally important for innate and/or acquired thermotolerance, we combined the use of a barcoded pool of ∼4,800 nonessential, prototrophic Saccharomyces cerevisiae deletion strains with Illumina-based deep-sequencing technology. As reported in other recent studies that have used deletion mutants to study stress responses, we observed that gene deletions resulting in the highest thermosensitivity generally are not the same as those transcriptionally induced in response to heat stress. Functional analysis of identified genes revealed that metabolism, cellular signaling, and chromatin regulation play roles in regulating thermotolerance and in acquired thermotolerance. However, for most of the genes identified, the molecular mechanism behind this action remains unclear. In fact, a large fraction of identified genes are annotated as having unknown functions, further underscoring our incomplete understanding of the response to heat shock. We suggest that survival after heat shock depends on a small number of genes that function in assessing the metabolic health of the cell and/or regulate its growth in a changing environment.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.1318100110