In vitro susceptibility of isolates of Sporothrix schenckii to terbinafine and itraconazole

The study objective was to determine the in vitro activity of terbinafine and itraconazole through the microdilution technique in broth (NCCLSM27-A2), adapted for dimorphic fungus, in relation to 12 isolates of Sporothrix schenckii. Six were from feline sporotrichosis, three from human sporotrichosi...

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Published in:Revista da Sociedade Brasileira de Medicina Tropical 2007-01, Vol.40 (1), p.60
Main Authors: Meinerz, Ana Raquel Mano, Nascente, Patrícia da Silva, Schuch, Luiz Filipe Damé, Cleff, Marlete Brum, Santin, Rosema, Brum, Cristiane da Silva, Nobre, Márcia de Oliveira, Meireles, Mario Carlos Araújo, Mello, João Roberto de Braga
Format: Article
Language:por ; eng
Subjects:
Animals
Antifungal Agents - pharmacology
Cats
Dogs
Humans
Itraconazole - pharmacology
Microbial Sensitivity Tests - methods
Naphthalenes - pharmacology
Sporothrix - drug effects
Sporothrix - isolation & purification
Terbinafine
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Summary:The study objective was to determine the in vitro activity of terbinafine and itraconazole through the microdilution technique in broth (NCCLSM27-A2), adapted for dimorphic fungus, in relation to 12 isolates of Sporothrix schenckii. Six were from feline sporotrichosis, three from human sporotrichosis, one from a dog and two from human isolates originating from Instituto Oswaldo Cruz. The inoculum and antifungal concentrates were distributed on microplates that were incubated at 35 degrees C for five days. Minimum inhibitory concentration readings were made at the end of this period. The MIC for terbinafine ranged from 0.055 microg/ml to 0.109 microg/ml, and the MIC for itraconazole ranged from 0.219 microg/ml to 1.75 microg/ml. For both drugs, the MIC from the isolates from IOC was 0.875 microg/ml. The present study demonstrates the high susceptibility of Sporothrix schenckii to terbinafine. Further studies to correlate the in vitro susceptibility tests with the clinical response of patients with sporotrichosis are needed.
ISSN:0037-8682
1678-9849
DOI:10.1590/S0037-86822007000100012