Chromosome aberrations in normal human fibroblasts analyzed in G^sub 0^/G^sub 1^ and G^sub 2^/M phases after exposure in G^sub 0^ to radiation with different linear energy transfer (LET)

We have studied the induction of chromosome aberrations in human fibroblasts exposed in G0/G1 to X-rays or heavy ions to study the influence of G1 cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were...

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Bibliographic Details
Published in:Mutation research. Genetic toxicology and environmental mutagenesis 2013-08, Vol.756, p.101
Main Authors: Liu, Cuihua, Kawata, Tetsuya, Furusawa, Yoshiya, Zhou, Guangming, Inoue, Kohei, Fukada, Junichi, Kota, Ryuichi, George, Kerry, Cucinotta, Francis, Okayasu, Ryuichi
Format: Article
Language:English
Subjects:
Biochemistry
Cellular biology
Chromosomes
X-rays
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Summary:We have studied the induction of chromosome aberrations in human fibroblasts exposed in G0/G1 to X-rays or heavy ions to study the influence of G1 cell cycle arrest. Confluent normal fibroblasts were exposed to X-rays or accelerated particles with different LET values and chromosome aberrations were investigated in the first G0/G1 and G2//M phase. The particles used here were 490 MeV/nucleon Si, 500 MeV/nucleon Fe, and 200 MeV/nucleon Fe ions. Cells were subcultured 24 h after exposure and premature chromosome condensation (PCC) was performed by fusion-induced method for analysis of G0/G1 cells, and chemically-induced method for analysis of G2 and metaphase cells. Chromosome damage was assessed in chromosomes 1 and 3 using whole chromosome fluorescence in situ hybridization (FISH). Cell cycle was analyzed by flow cytometry at different incubation times following subculture. After irradiation with 2 Gy of high-LET particles, the yields of chromosome aberrations and fragments were significantly higher in G0/G1 phase than in G2/M phase, whereas similar yields of damage were measured in both phases after exposure to X-rays. In contrast, the yield of misrepair, assessed by the number of color junctions, was similar in the G0/G1 and G2/M phases after exposure to either X-rays or high-LET particles. The yields of chromosome aberrations, fragments, and color junctions in both the G0/G1 and the G2/M phases, increased with LET up to 200 keV/...m, then decreased for 440 keV/...m Fe particles. A good correlation was found between chromosome aberrations in both G0/G1 and G2/M cells and survival fractions after 2 Gy of different LET radiations, although the slopes were steeper for the G0/G1 cells. Flow cytometry analysis indicated that high-LET particles induce more non cycling G0/G1 cells within 48 h of subculture than X-rays, suggesting that chromosome aberrations scored at the G2/M phase may not accurately describe the true radiation effect. (ProQuest: ... denotes formulae/symbols omitted.)
ISSN:1383-5718
1879-3592