Protective effect of cyanidin-3-O-[beta]-D-glucopyranoside fraction from mulberry fruit pigment against oxidative damage in streptozotocin-induced diabetic rat bladder

Aims To determine whether cyanidin-3-O-[beta]-D-glucopyranoside (C3G) fraction from mulberry fruit pigment has protective effects against bladder dysfunction on streptozotocin-induced diabetic rats Methods Sprague-Dawley rats were divided into three groups (n=12 in each): normal, diabetes (DM), and...

Full description

Saved in:
Bibliographic Details
Published in:Neurourology and urodynamics 2013-06, Vol.32 (5), p.493
Main Authors: Ha, U-Syn, Bae, Woong-Jin, Kim, Su-Jin, Yoon, Byung-Il, Jang, Hoon, Hong, Sung-Hoo, Lee, Ji-Yeoul, Hwang, Seung-Yeon, Kim, Sae-Woong
Format: Article
Language:English
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Aims To determine whether cyanidin-3-O-[beta]-D-glucopyranoside (C3G) fraction from mulberry fruit pigment has protective effects against bladder dysfunction on streptozotocin-induced diabetic rats Methods Sprague-Dawley rats were divided into three groups (n=12 in each): normal, diabetes (DM), and DM treated with C3G fraction (DM+C3G). The DM and DM+C3G groups received a single injection of streptozotocin (50mg/kg) intraperitoneally. Four weeks after the induction of diabetes, the DM+C3G group was treated with daily oral C3G (10mg/kg) dissolved in water, for 8 weeks. After 12 weeks of streptozotocin injections, rats in each group underwent cystometrography and bladders were used for evaluation of apoptosis and oxidative stress. Results The DM group showed a markedly lower maximal intravesical pressure than that observed in the control group, whereas rats in the DM+C3G group showed improved maximum intravesical pressure associated with minimization of apoptosis, and increased levels of Akt and Bad phosphorylation, implying inhibition of pro-apoptotic stimuli. The level of 8-hydroxy-2-deoxyguanosine, a marker of oxidative stress, was significantly greater in the DM group compared to the control group and it was significantly reduced in the C3G treated group. Immunoblotting revealed a significant decrease in the levels of the superoxide dismutase protein and nerve growth factor in the DM group compared with the control group; however, these proteins were upregulated in the DM+C3G group compared with the DM group. Conclusions The study is the first to suggest that C3G fraction have a potency to protect the bladder under conditions of diabetes-induced oxidative stress. Neurourol. Urodynam. 32: 493-499, 2013. © 2012 Wiley Periodicals, Inc. [PUBLICATION ABSTRACT]
ISSN:0733-2467
1520-6777
DOI:10.1002/nau.22334