Tonoplast of Beta vulgaris L. contains detergent-resistant membrane microdomains

The experiments conducted on tonoplast of Beta vulgaris L. roots were performed to identify detergent-resistant lipid—protein microdomains (DRMs, interpreted as lipid rafts).The presence of DRMs can be found when dynamic clustering of sphingolipids, sterols, saturated fatty acids is registered, and...

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Bibliographic Details
Published in:Planta 2013-03, Vol.237 (3), p.859-871
Main Authors: Ozolina, Natalia V., Nesterkina, Irina S., Kolesnikova, Ekaterina V., Salyaev, Ryurik K., Nurminsky, Vadim N., Rakevich, Alexander L., Martynovich, Evgueni F., Chernyshov, Michael Yu
Format: Article
Language:English
Subjects:
2-Naphthylamine - analogs & derivatives
2-Naphthylamine - metabolism
Agriculture
Beets
Beta vulgaris - drug effects
Beta vulgaris - metabolism
Biomedical and Life Sciences
Cell membranes
Centrifugation, Density Gradient
Detergents - pharmacology
Ecology
Enzyme Inhibitors - pharmacology
Fatty acids
Fatty Acids - metabolism
Fluorescence
Forestry
Intracellular Membranes - drug effects
Intracellular Membranes - metabolism
Laurates - metabolism
Life Sciences
Lipids
Membrane Microdomains - drug effects
Membrane Microdomains - metabolism
Original Article
Plant cells
Plant Proteins - metabolism
Plant Sciences
Proton-Translocating ATPases - antagonists & inhibitors
Proton-Translocating ATPases - metabolism
Rafts
Sterols
Sterols - metabolism
Tonoplast
Vacuoles
Vacuoles - drug effects
Vacuoles - metabolism
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Summary:The experiments conducted on tonoplast of Beta vulgaris L. roots were performed to identify detergent-resistant lipid—protein microdomains (DRMs, interpreted as lipid rafts).The presence of DRMs can be found when dynamic clustering of sphingolipids, sterols, saturated fatty acids is registered, and the insolubility of these microdomains in nonionic detergents at low temperatures is proven. The elucidation of tonoplast microdomains has been based on results obtained with the aid of high-speed centrifuging in the sucrose gradient. The experiments have shown that tonoplast microdomains are rich in sphingolipids, free sterols and saturated fatty acids (such a lipid content is also typical of lipid—protein microdomains of other membranes), while only few phospholipids are present in tonoplast microdomains. The presence of microdomains has been confirmed by fluorescence and confocal microscopy using filipin and Laurdan as fluorescent probes. The experiments with Laurdan have shown that tonoplast microdomains are characterized by a high order compared to characteristics of the rest of the tonoplast. Thus, the presence of detergent-resistant lipid—protein microdomains in the tonoplast has been demonstrated.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-012-1800-1