Differential responses of antioxidative defense system to prolonged salinity stress in salt-tolerant and salt-sensitive Indica rice (Oryza sativa L.) seedlings

The present investigation evaluated the ability of an antioxidative defense system in terms of the tolerance against salinity-induced oxidative stress and also explored a possible relationship between the status of the components of an antioxidative defense system and the salt tolerance in Indica ri...

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Bibliographic Details
Published in:Protoplasma 2013-02, Vol.250 (1), p.3-19
Main Authors: Mishra, Pallavi, Bhoomika, Kumari, Dubey, R. S
Format: Article
Language:English
Subjects:
antioxidant activity
antioxidants
Antioxidants - metabolism
ascorbate peroxidase
Biomedical and Life Sciences
catalase
Cell Biology
cultivars
genotype
glutathione
glutathione dehydrogenase (ascorbate)
glutathione-disulfide reductase
hydrogen peroxide
Life Sciences
lipid peroxidation
monodehydroascorbate reductase (NADH)
Original Article
Oryza - enzymology
Oryza - metabolism
Oryza - physiology
Oryza sativa
oxidation
oxidative stress
Oxidative Stress - physiology
peroxidase
Plant Sciences
rice
Salinity
salt stress
Salt-Tolerance
Salt-Tolerant Plants
seedlings
Seedlings - metabolism
sodium chloride
Stress, Physiological - physiology
superoxide anion
superoxide dismutase
Superoxides - metabolism
thiobarbituric acid-reactive substances
thiols
Zoology
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Summary:The present investigation evaluated the ability of an antioxidative defense system in terms of the tolerance against salinity-induced oxidative stress and also explored a possible relationship between the status of the components of an antioxidative defense system and the salt tolerance in Indica rice (Oryza sativa L.) genotypes. When the seedlings of a salt-sensitive cultivar was grown in sand cultures containing different NaCl concentrations (7 and 14 dS m⁻¹) for 5–20 days, a substantial increase was observed in the rate of superoxide anion (O ₂ ·⁻ ) production, elevated levels of H₂O₂ and thiobarbituric acid reactive substances (TBARS) which indicated an enhancement in lipid peroxidation. A declination in the level of thiol clearly indicated an increase in the protein oxidation as well as a decline in the reduced forms of ascorbate (AsA) and glutathione (GSH) and the ratios of their reduced to oxidized forms occurred in the salt-sensitive seedlings. Similar treatment caused a very little alteration or no change in the levels of these components in the seedlings of salt-tolerant cultivar. The activity of antioxidative enzymes superoxide dismutase (SOD), its isoform Cu/Zn-SOD and ascorbate peroxidase (APX) increased in both the cultivars against salinity. In salt-sensitive seedlings, the activity of the various enzymes, guaiacol peroxidase (GPX), catalase (CAT), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), and glutathione reductase (GR) increased at moderate salinity treatment of 7 dS m⁻¹ NaCl while the activities of these enzymes declined with higher salinity level of 14 dS m⁻¹ NaCl. However, a consistent increase was observed in the activities of these enzymes of salt-tolerant seedlings with an increase in the duration and the level of the salinity treatment. The results suggest that a higher status of antioxidants (AsA and GSH) and a coordinated higher activity of the enzymes (SOD, CAT, GPX, APX, and GR) can serve as the major determinants in the model for depicting salt tolerance in Indica rice seedlings.
ISSN:0033-183X
1615-6102
DOI:10.1007/s00709-011-0365-3