Loading…
Microtubule stability affects the unique motility of F-actin in Marchantiapolymorpha
Actin microfilaments play crucial roles in diverse plant functions. Some specific cellular processes require interaction between F-actin and microtubules, and it is believed that there are direct or indirect connections between F-actin and microtubules. We previously reported that actin microfilamen...
Saved in:
Published in: | Journal of plant research 2013, Vol.126 (1), p.113-119 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Actin microfilaments play crucial roles in diverse plant functions. Some specific cellular processes require interaction between F-actin and microtubules, and it is believed that there are direct or indirect connections between F-actin and microtubules. We previously reported that actin microfilaments exhibit unique dynamic motility in cells of the liverwort,
Marchantia polymorpha
; the relevance of this activity to microtubules has not been explored. To examine whether the dynamics of F-actin in
M. polymorpha
were somehow regulated by microtubules, we investigated the effects of stabilization or destabilization of microtubules on dynamics of actin bundles, which were visualized by Lifeact-Venus. To our surprise, both stabilization and destabilization of microtubules exerted similar effects on F-actin motility; apparent sliding movement of F-actin in
M. polymorpha
cells was accelerated by both oryzalin and paclitaxel, with the effect of paclitaxel more evident than that of oryzalin. Immunofluorescence staining revealed that some F-actin bundles were arrayed along with microtubules in
M. polymorpha
thallus cells. These results suggest that microtubules play regulatory roles in the unique F-actin dynamics in
M. polymorpha
. |
---|---|
ISSN: | 0918-9440 1618-0860 |
DOI: | 10.1007/s10265-012-0496-4 |