cDNA cloning and expression of proliferating cell nuclear antigen (PCNA) in white leg shrimp (Litopenaeus vannamei) challenged with Vibrio anguillarum

In present investigation the proliferating cell nuclear antigene (PCNA) of white leg shrimp (Litopenaeus vannamei) (LvPCNA) has been cloned by rapid amplification of cDNA (RACE) and anchored PCR method. The full length LvPCNA had 1131 bp containing 786 bp of open reading frame (ORF), encoding 261 am...

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Bibliographic Details
Published in:Pakistan journal of zoology 2012, Vol.44 (4), p.1029-1034
Main Authors: Muhammad, F. (Karachi Univ. (Pakistan). Centre of Excellence in Marine Biology), Ming-Yu, S. (Ocean Univ. of China, Qingdao (China). Ministry of Education), Xioli, S. (Ocean Univ. of China, Qingdao (China). Ministry of Education), Shafi, M. (Ocean Univ. of China, Qingdao (China). Ministry of Education), Xiao-Ling, L. (Yantai Univ., Yantai (China). Coll. of Life Sciences)
Format: Article
Language:English
Subjects:
ANTIGENS
MOLECULAR CLONING
PATHOGENS
PCR
PENAEUS VANNAMEI
PRAWNS AND SHRIMPS
VIBRIO ANGUILLARUM
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Summary:In present investigation the proliferating cell nuclear antigene (PCNA) of white leg shrimp (Litopenaeus vannamei) (LvPCNA) has been cloned by rapid amplification of cDNA (RACE) and anchored PCR method. The full length LvPCNA had 1131 bp containing 786 bp of open reading frame (ORF), encoding 261 amino acids with an estimating molecular weight of 28.8 kDa. Tissue distribution of the LvPCNA after real time analysis revealed that expression is high in gill and muscle. The expression level after bacterial challenge of LvPCNA in hepatopancreas suggested the LvPCNA might be involved in defense mechanism of shrimp. The present investigation is a contribution to the existing knowledge of immune responses of shrimp to bacterial challenge.
ISSN:0030-9923