Bioinspired multivalent DNA network for capture and release of cells

Capture and isolation of flowing cells and particulates from body fluids has enormous implications in diagnosis, monitoring, and drug testing, yet monovalent adhesion molecules used for this purpose result in inefficient cell capture and difficulty in retrieving the captured cells. Inspired by marin...

Full description

Saved in:

Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS 2012-11, Vol.109 (48), p.19626-19631
Main Authors:	Zhao, Weian, Cui, Cheryl H, Bose, Suman, Guo, Dagang, Shen, Chong, Wong, Wesley P, Halvorsen, Ken, Farokhzad, Omid C, Teo, Grace Sock Leng, Phillips, Joseph A, Dorfman, David M, Karnik, Rohit, Karp, Jeffrey M
Format:	Article
Language:	eng
Subjects:	adhesion antibodies Binding Sites Biological Sciences Blood Blood cells Body fluids Cell Line Cells Deoxyribonucleic acid DNA DNA - metabolism DNA - physiology drugs Epithelial cells Flow velocity gene overexpression Humans Microfluidics Molecules monitoring neoplasm cells neoplasms oligonucleotides particulates Physical Sciences Proteins Shear stress Stem cells T lymphocytes tyrosine
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Capture and isolation of flowing cells and particulates from body fluids has enormous implications in diagnosis, monitoring, and drug testing, yet monovalent adhesion molecules used for this purpose result in inefficient cell capture and difficulty in retrieving the captured cells. Inspired by marine creatures that present long tentacles containing multiple adhesive domains to effectively capture flowing food particulates, we developed a platform approach to capture and isolate cells using a 3D DNA network comprising repeating adhesive aptamer domains that extend over tens of micrometers into the solution. The DNA network was synthesized from a microfluidic surface by rolling circle amplification where critical parameters, including DNA graft density, length, and sequence, could readily be tailored. Using an aptamer that binds to protein tyrosine kinase-7 (PTK7) that is overexpressed on many human cancer cells, we demonstrate that the 3D DNA network significantly enhances the capture efficiency of lymphoblast CCRF-CEM cells over monovalent aptamers and antibodies, yet maintains a high purity of the captured cells. When incorporated in a herringbone microfluidic device, the 3D DNA network not only possessed significantly higher capture efficiency than monovalent aptamers and antibodies, but also outperformed previously reported cell-capture microfluidic devices at high flow rates. This work suggests that 3D DNA networks may have broad implications for detection and isolation of cells and other bioparticles.
ISSN:	0027-8424 1091-6490