Distribution of macrophages during fish development: an immunohistochemical study in carp (Cyprinus carpio, L.)

A monoclonal antibody against carp macrophages (WCL15) has been utilised in flow cytometry, immuno-histochemistry and immuno-electron microscopy to assess the distribution of monocytes/macrophages in developing carp lymphoid tissues. In suspensions of living cells WCL15 reacted strongly with cytopla...

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Bibliographic Details
Published in:Anatomy and Embryology 1998-07, Vol.198 (1), p.31-41
Main Authors: Romano, N, Picchietti, S, Taverne-Thiele, J J, Taverne, N, Abelli, L, Mastrolia, L, Verburg-van Kemenade, B M, Rombout, J H
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Carp
Carps - embryology
Cell Count
Embryo, Nonmammalian - cytology
Embryonic Development
Female
Fish
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Immunoenzyme Techniques
Lymphocyte receptors
Lymphoid Tissue - cytology
Lymphoid Tissue - growth & development
Macrophages - cytology
Male
Microscopy, Immunoelectron
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Summary:A monoclonal antibody against carp macrophages (WCL15) has been utilised in flow cytometry, immuno-histochemistry and immuno-electron microscopy to assess the distribution of monocytes/macrophages in developing carp lymphoid tissues. In suspensions of living cells WCL15 reacted strongly with cytoplasm and plasmic membrane of macrophages. It also cross-reacted with a subpopulation of thrombocytes, but this reaction could be neglected by double immunostaining in combination with a thrombocyte-specific marker. In Bouin-fixed tissues the antibody distinctly recognised macrophages. Macrophages were found from day 2 post-fertilisation in head kidney and in the dorsal portion of the yolk sac epithelium. From 1 week onwards macrophages were found scattered in thymus and gut and during the second week in spleen. Macrophages increased in number in all lymphoid tissues until the 6-8th week post-fertilisation, but they decreased except in thymus, where they became localised mainly in the cortical-medullary boundary, and in white pulp areas of head kidney. The role of macrophages in allowing an early non-specific defence in young fish and in co-operating during the differentiation processes of T-cells and B-cells is discussed.
ISSN:0340-2061
1863-2653
1432-0568
0340-2061
DOI:10.1007/s004290050162