Antioxidant activity of phenolic compounds identified in sunflower seeds

The antioxidant activity and phenolic compound profiles of six fractions (I–VI) obtained from sunflower seed extract were studied. HPLC–MS(ESI) analysis was applied for quantitative and qualitative determination of phenolic compounds of the fractions. The antioxidant activity of the fractions was st...

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Bibliographic Details
Published in:European food research & technology 2012-08, Vol.235 (2), p.221-230
Main Authors: Karamać, Magdalena, Kosińska, Agnieszka, Estrella, Isabel, Hernández, Teresa, Dueñas, Montserrat
Format: Article
Language:English
Subjects:
Acids
Agriculture
Alzheimer's disease
Analytical Chemistry
Antioxidants
Biological and medical sciences
Biotechnology
Cellulose acetate
Chemistry
Chemistry and Materials Science
Chromatography
Food
Food industries
Food Science
Forestry
Fundamental and applied biological sciences. Psychology
Original Paper
Oxidation
Seeds
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Summary:The antioxidant activity and phenolic compound profiles of six fractions (I–VI) obtained from sunflower seed extract were studied. HPLC–MS(ESI) analysis was applied for quantitative and qualitative determination of phenolic compounds of the fractions. The antioxidant activity of the fractions was studied in terms of their ability to scavenge DPPH · and ABTS ·+ and to reduce Fe 3+ /ferricyanide complex to the ferrous form and was expressed as EC 50 , TEAC and reducing power values, respectively. The results of all antioxidant activity tests showed good correlations among each other and with the phenolic contents for the individual fractions. The fractions IV–VI were characterized by high antioxidant activity. 5- O -Caffeoylquinic acid was a predominant compound of fractions IV and V, while dicaffeoylquinic acid isomers and caffeoyl-dimethoxycinnamoylquinic acid isomers accounted for 76.6 % of phenolic compounds of fraction VI. Ferulic acid, p -coumaroylquinic acid isomers, ferulic acid dehydrotrimer isomers and some quercetin derivatives were also identified. The highest content of those compounds was noted in fraction III.
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-012-1751-6