Building Highly Sensitive Dye Assemblies for Biosensing from Molecular Building Blocks

Fluorescence superquenching is investigated for polyelectrolytes consisting of cyanine dye pendant polylysines ranging in number of polymer repeat units (NPRU) from 1 to 900, both in solution and after adsorption onto silica nanoparticles. As NPRUincreases, the absorption and fluorescence evolve fro...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-12, Vol.98 (26), p.14769-14772
Main Authors: Jones, Robert M., Lu, Liangde, Helgeson, Roger, Bergstedt, Troy S., McBranch, Duncan W., Whitten, David G.
Format: Article
Language:English
Subjects:
Absorption spectra
Aggregation
Biosensing Techniques
Dyes
Excitons
Fluorescence
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Microspheres
Molecular biology
Monomers
Nanoparticles
Oligomers
Physical Sciences
Polymers
Rapid quenching
Spectrometry, Fluorescence
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Summary:Fluorescence superquenching is investigated for polyelectrolytes consisting of cyanine dye pendant polylysines ranging in number of polymer repeat units (NPRU) from 1 to 900, both in solution and after adsorption onto silica nanoparticles. As NPRUincreases, the absorption and fluorescence evolve from monomer spectra to red-shifted features indicative of molecular J aggregates. In solution, the superquenching sensitivity toward an anionic electron acceptor increases by more than a millionfold over the NPRUrange from 1 to 900. The dramatic increase is attributed to enhanced equilibrium constants for binding the quenchers, and the amplified quenching of a delocalized exciton of ≈100 polymer repeat units. The self-assembly of monomer onto silica and clay nanoparticles leads to formation of J aggregates, and surface-activated superquenching enhanced 10,000× over the monomer in solution, indicating the formation of "self-assembled polymers" on the nanoparticle surface. Utilization of these self-assembled polymers as high-sensitivity biosensors is demonstrated.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.251555298