Interaction between Inducible Nitric Oxide Synthase and Cyclooxygenase-2 after Cerebral Ischemia

Focal cerebral ischemia is associated with expression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), enzymes whose reaction products contribute to the evolution of ischemic brain injury. We tested the hypothesis that, after cerebral ischemia, nitric oxide (NO) produced...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1998-09, Vol.95 (18), p.10966-10971
Main Authors: Nogawa, Shigeru, Forster, Colleen, Zhang, Fangyi, Nagayama, Masao, Ross, M. Elizabeth, Iadecola, Costantino
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological Sciences
Brain
Brain - enzymology
Brain - metabolism
Brain ischemia
Brain Ischemia - enzymology
Brain Ischemia - metabolism
Cerebral cortex
Cyclooxygenase 2
Dinoprostone - metabolism
DNA Primers
Enzymes
Gases
Ischemia
Isoenzymes - genetics
Isoenzymes - metabolism
Messenger RNA
Mice
Mice, Inbred C57BL
Neurons
Neutrophils
Nitric Oxide Synthase - genetics
Nitric Oxide Synthase - metabolism
Nitric Oxide Synthase Type II
Olfactory bulb
Pharmacology
Prostaglandin-Endoperoxide Synthases - genetics
Prostaglandin-Endoperoxide Synthases - metabolism
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
Vehicles
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Summary:Focal cerebral ischemia is associated with expression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), enzymes whose reaction products contribute to the evolution of ischemic brain injury. We tested the hypothesis that, after cerebral ischemia, nitric oxide (NO) produced by iNOS enhances COX-2 activity, thereby increasing the toxic potential of this enzyme. Cerebral ischemia was produced by middle cerebral artery occlusion in rats or mice. Twenty-four hours after ischemia in rats, iNOS-immunoreactive neutrophils were observed in close proximity (
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.95.18.10966